Tìm kiếm

Kết quả Tìm kiếm

• IMMUNO-RELATED GENE EXPRESSION OF ANTI-LIPOPOLYSACCHARIDE FACTORS (ALF) AFTER USING IMMUNOSTIMULANT IN KURUMA SHRIMP (Marsupenaeus japonicus)

  Vu Duc Hanh, Lai Thi Lan Huong

  Ngày nhận bài: 31-07-2025 / Ngày xuất bản: 06-08-2025

   Tóm tắt PDF

  Supplementation of immuno-stimulant can act as a potent immune modulator and have the effect on inducion of immune- related genes which involved in host defense. In order to clarify the anti-lipopolysaccharide factors (ALF) expression of Kuruma shrimp (Marsupenaeus japonicus) after feeding immuno-stimulant, the supplement containing microalgae and Euglena (Ex400) was selected for the trials. Total RNA was extracted by RNA Iso Plus from the lymphoid organ, intestine and blood of shrimp that was fed with Ex400 diet. Tissues were collected at 0, 3, 7, and 10 days after feeding. ALF transcripts were significantly increased by Ex400 compared to control-diet-fed shrimp (with P<0.01) at 3 days post-feeding in the intestine and lymphoid organ. This suggested Ex test diet might stimulate ALF of Kuruma shrimp immune defence in the intestine and lymphoid organ.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.9.

• EXPRESSION OF IMMUNO-RELATED GENE (LYSOZYME) AFTER USING IMMUNOSTIMULANT IN KURUMA SHRIMP (Marsupenaeus japonicus)

  Vu Duc Hanh, Nguyen Thi Hong Chien, Dao Le Anh, Nguyen Thi Hang, Le Van Truong, T. Itami, Lai Thi Lan Huong, Nguyen Thi Lan

  Ngày nhận bài: 26-07-2025 / Ngày xuất bản: 06-08-2025

   Tóm tắt PDF

  Immunostimulants are the substances, which enhance the immuno-defense activity and provide resistance to fish and shrimp diseases. In order to clarify the lysozyme expression of kuruma shrimp (Marsupenaeus japonicus) after feeding immunostimulant, the supplement containing microalgae and Euglena (Ex400) were selected for the trials. Total RNA was extracted by RNA Iso Plus from the lymphoid organ, intestine and blood of shrimp that was fed with Ex400 diet. Tissues were sampled at 0, 3, 7, and 10 days after feeding. Lysozyme transcripts significantly increased in Ex400-fed shrimps compared to control diet-fed shrimps (P < 0.01) at 7 and 10 days post-feeding in lymphoid organ and intestine. Lysozyme expression of blood of shrimp also exhibited higher level than those of control at 3 and 10 days post feeding (P < 0.01). In conclusion, the result of lysozyme gene expression suggested that immunostimulant could activate lysozyme in the immune system of kuruma shimp.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.3.