Ngày nhận bài: 26-07-2025
Ngày xuất bản: 06-08-2025
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Cách trích dẫn:
EXPRESSION OF IMMUNO-RELATED GENE (LYSOZYME) AFTER USING IMMUNOSTIMULANT IN KURUMA SHRIMP (Marsupenaeus japonicus)
,
Nguyen Thi Hong Chien
1, 2
,
Dao Le Anh
1, 2
,
Nguyen Thi Hang
1, 2
,
Le Van Truong
1, 2
,
T. Itami
1, 2
,
Lai Thi Lan Huong
1, 2
,
Nguyen Thi Lan
1, 2
Từ khóa
Gene expression, immunostimulants, kuruma shrimp, lysozyme, quantitative real-time PCR
Tóm tắt
Immunostimulants are the substances, which enhance the immuno-defense activity and provide resistance to fish and shrimp diseases. In order to clarify the lysozyme expression of kuruma shrimp (Marsupenaeus japonicus) after feeding immunostimulant, the supplement containing microalgae and Euglena (Ex400) were selected for the trials. Total RNA was extracted by RNA Iso Plus from the lymphoid organ, intestine and blood of shrimp that was fed with Ex400 diet. Tissues were sampled at 0, 3, 7, and 10 days after feeding. Lysozyme transcripts significantly increased in Ex400-fed shrimps compared to control diet-fed shrimps (P < 0.01) at 7 and 10 days post-feeding in lymphoid organ and intestine. Lysozyme expression of blood of shrimp also exhibited higher level than those of control at 3 and 10 days post feeding (P < 0.01). In conclusion, the result of lysozyme gene expression suggested that immunostimulant could activate lysozyme in the immune system of kuruma shimp.
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