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• Development of China Pink (Dianthus chinensis) Polyploid Lines via In Vitro Treatment with Colchicine

  Nguyen Thi Ly Anh, Nguyen Thi Thanh Phuong, Ho Thi Thu Thanh, Le Hai Ha, Nguyen Thi Han

  Date Received: 16-10-2014 / Date Accepted: 24-11-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  Colchicine has been successfully used for in vitro treatment for inducton of polyploids in many plants with high efficiency. The aim of this study was to generate new polyploid lines of China pink for breeding and selection in Vietnam. The experiments were carried out on the cultivar Tim Vien Trang of China pink (Dianthus chinensis). In vitro nodal stems were used for treatment with colchicine solution atconcentration of 0, 0.01%, 0.05% and 0.1%  for 24 hours, 48 hours and 72 hours. The mutant types were isolated based on morphological characteristics in in vitro and in vivo conditions. The results showed that the ploidy mutation rate was in a range of 0.87% to 12.85% when the explants were treated in 0.05% and 0.1% of colchicine solution for 24 hours and 48 hours and in 0.01% and 0.05% of colchicine solution for 72 hours. The mutants had highest quality from the treatments of 0.05% and 0.1% of colchicine concentration for 48 hours. Among eight different mutant types, the mutant types D7 and D9 carried several superior morphosological features such as the plant height, leaf size, flower diameter, flower longevity and color intensity in comparison with original cultivar. These two polyploid lines were cloned in vitro for further evaluation of genetic stability.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.8.

• In vitro Micropropagation of Dendrobium officinale Kimura et Migo

  Nguyen Thi Son, Tu Bich Thuy, Dang Thi Nhan, Nguyen Thi Ly Anh, Hoang Thi Nga, Nguyen Quang Thach

  Date Received: 16-10-2014 / Date Accepted: 24-11-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

   Dendrobium officinale Kimura et Migo. is a precious orchid and has been using as medicinal plant. Moreever, this plant has been used to produce functional foods for treatment of diabetes and difficult-to-cure diseases, which are commercially available world-wide. In vitro propagation of D. officinale Kimura et Migo was conducted in order to maintain the genetic pool of this precious orchid species. The results showed that the medium for in vitro seed germination was VW + 10g sucrose + 6g agar + 100ml coconut milk per liter. The best medium for rapid propagation of shoot cluster was MS + 20g sucrose + 100ml coconut milk + 6g agar + 0.5 mg BA + 60g ripe banana per liter. The most appropriate medium for propagation of in vitro nodal stems was MS + 20g sucrose + 100ml coconut milk+ 0.5mg/l α-NAA + 6g agar per liter. The rooting medium was RE + 10g sucrose + 6g agar + 0.3g active charcoal + 0.5 mg/l α-NAA per liter.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.8.