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• Effects of Salinity on The Growth of Nannochloropsis oculata in In Vitro Culture

  Trinh Cam Tu, Tran Thanh Huong, Bui Trang Viet

  Date Received: 07-08-2014 / Date Accepted: 24-09-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  Nannochloropsis oculata grown in modified f/2 medium exhibited a double sigmoid growth curve with four phases: two phases of rapid growth separated by an intermediate slow growth phase, and a saturated phase. In the rapid growth phases, microalgal cells divided rapidly and achieved 2x107 cells per mL on the sixth day. The slow growth phase occurred with the increase of cell diameter and dry weight. The last phase started from the twelfth day, in which there was little or no growth. Effects of sodium chloride on N. oculata cell division depended on concentration and time of treatment. The removal of sodium chloride (NaCl 0M) or the increase of sodium chloride (NaCl 2M) in ten hours from modified f/2 medium yielded in the increase in cell division. Besides, the extension of treatment time from ten to twenty hours resulted in decrease of sodium chloride effects.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.7.

• Using Cotyledon as a Material for Studying the Effect of Sodium Chloride on Mimosapigra L.Leaf Photosynthesis

  Dõ Thuòng Kiẹt, Thomas J Givnish, Tran Triet, Bùi Trang Viẹt

  Date Received: 10-09-2012 / Date Accepted: 18-12-2012

   Abstract PDF (VIETNAMESE)

  Sodium chloride (NaCl) was used as an alternative herbicide to control some low shrub weeds. Mimosa pigra leaves turned brown when sprayed with NaCl. Two-days-old cotyledons of Mimosa pigra L. are capable of photosynthesis similar to mature leaflets and were used to study the morphological changes as well as photosynthesis after NaCl treatment. The results showed that NaCl at high concentrations of 10 to 60 g/l caused the loss of chlorophyll, carotenoid, leading to the loss of green color and browning of mesophyll tissue in cotyledon. Photoinhibition in Mimosa pigra L. cotyledon caused by NaCl occured only when it was exposed to strong light and reflected by a decrease in the value of Fv/Fm, QP, QN and electron transport chain of cotyledon. The rate of oxygen emission as well as carbon dioxide assimilation of cotyledon decreased as NaCl concentration increased.

• Effect of Sodium Chloride on Morphological Changes and Gas Exchange of Mimosapigra L. Leaf

  Dõ Thuòng Kiẹt, Tran Triet, Bùi Trang Viẹt

  Date Received: 01-10-2012 / Date Accepted: 30-10-2012 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  A new chemical control method has been applied on Mimosa pigra L., one of the most dangerous weeds in the world, to control this species in a safer way with less environmental pollution. NaCl at different concentrations were applied directly on the leaves or secondary leaflets of this plant. The results showed that NaCl caused the browning on Mimosa pigra L. leaves, starting from the tip of the secondary leaflet, spreading to the base. Browning area increased over time after treatment and with the increase of NaCl concentration. The leaflets browning appeared after 24 hours and completely damaged after 2 days of NaCl 30 g/l treatment. NaCl 30 g/l caused the loss of chlorophyll, primary parenchymal cells plasmolysis, stomatal closing, and death after 24 hours. The loss of chlorophyll in chloroplasts appeared firstly at palisade parenchyma tissue and spread to other parenchyma tissue below. This was the original browning caused by NaCl on Mimosa pigra L. leaf.

  DOI: https://doi.org/10.31817/tckhnnvn.2012.10.6.

• Phân lập, tuyển chọn và định danh Aspergillus oryzae có khả năng sinh protease trung tính và chịu mặn cao từ một số thực phẩm lên men truyền thống

  Vu Thi Lan, Nguyen Hoang Anh

  Date Received: 14-08-2017 / Date Accepted: 18-09-2017 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  Nghiên cứu này nhằm phân lập, tuyển chọn và định tên Aspergillus oryzae từ một số thực phẩm lên men truyền thống có khả năng sinh protease trung tính và chịu mặn cao, có tiềm năng ứng dụng trong lên men thực phẩm và các ứng dụng khác ở điều kiện muối cao. Mười hai trong 23 chủng được định danh sơ bộ là Aspergillus oryzae bằng phương pháp quan sát hình thái. Trong đó, chủng TB1 phân lập từ tương bần sinh protease cao nhất với 49,26 U/l, tương ứng với đường kính 17mm của vòng phân giải trên đĩa thạch chứa BCG được định danh bằng phương pháp sinh học phân tử và đặt tên là Aspergillus oryzae TB1. Protease của chủng này có hoạt độ cao trong khoảng pH 5,0 - 8,0 và tối ưu ở pH 7,0. Hoạt độ enzyme vẫn còn 70% sau 8 giờ ủ ở pH 7,0 và 37oC. Nhìn chung, hoạt độ protease giảm khi nồng độ muối natri clorua tăng từ 0 đến 16%, hoạt độ tương đối là 51,8% ở 16% NaCl và nồng độ muối này được sử dụng để xác định khả năng chịu mặn của protease. Kết quả cho thấy hoạt độ enzyme còn lại là 49,2% sau 9 giờ ủ ở 37oC.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.9.

• Analysis of Blood Haematological and Biochemical Parameters in Buffaloes (Bubalus bubalis) Raised in Cam Thuy District, Thanh Hoa Province

  Nguyen Ba Tiep, Luu Tuan Nghia

  Date Received: 09-06-2016 / Date Accepted: 23-11-2016 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  This study was done to determine blood haematological parameters (red blood cell count, hemoglobin, white blood cell count, white blood cell composition, platelet cell count and other platelet parameters) and blood biochemistry (total plasma proteins, serum albumin, globulin and glucose, serum sodium, serum postassium, serum chloride and calcium levels) of water buffaloes raised in Cam Thuy district, Thanh Hoa province. Red blood cell count, hemoglobin values, white blood cell count, platelet cell count, plasma total protein and globulin concentrations were age-dependent. Sex dependences were observed in red cell distribution width but not in white blood cell and platelet values. The determined values were compared with reference values of the International Federation of Clinical Chemistry (IFCC) and results obtained from previous studies. The present results can be employed as reference data for study, diagnosis and treatment of bufalo diseases with widely used automated analyzers for clinical blood parameters.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.11.