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• Technical Advances in Reproduction: Applications in Medicine and Agriculture

  Nguyen Van Thuan

  Date Received: 18-04-2017 / Date Accepted: 26-07-2017 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  In mammals, reproduction is the ability to generate next generation through the fertilization of a mature egg and a sperm. Nowadays, with the development of modern reproductive biotechnology, several advanced techniques have been applied and able to generate new generations from somatic cellsandstem cells or from immature eggs and sperms. In the present paper, the advances in reproduction over the past 20 yearsare sumarized and presented including: (1) intracytoplasmic sperm injection (ICSI) and application of this method to treat infertility and production of transgenic animals, (2) round spermatid injection (ROSI) and itsapplication on treatment of infertility without spermatozoa, (3) Somatic cell nuclear transfer (SCNT), also known as animal cloning, and (4) the method to generate new generation by pluripotent stem cells injected into tetraploid blastocysts.The application potential of the modern reproductive methods in medicine and agriculturewas discussed.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.7.

• Effect of Colchicine Concentration and Duration of Treatment on Polyploidy Induction in Shallot (Allium cepaL., Aggregatum Group)

  Tran Thi Minh Hang, Pham Thi Minh Phuong, Nguyen Thanh Tuan, Phan Thi Ngan

  Date Received: 09-11-2015 / Date Accepted: 11-03-2016 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  Local shallot (Allium cepaL. ), Aggregatum group) variety grown inKinh Mon, Hai Duong was treated with colchicine in order to produce polyploid materials for high-quality shallot breeding in Viet Nam. The research was carried out at Faculty of Agronomy, Vietnam National University of Agriculture. The shallot bulbs were immersedincolchicine solution with different concentrations (0,2%; 0,4%; 0,6%; 0,8%; 1,0%; 1,2%; 1,4%; 1.6%; 1,8%; 2,0%)for oneto tendays. The results revealed that colchicine treatment at concentration 1,0% for 6 days was most suitable for inducing tetraploid shallot plants with survival rate of 80%. Thepercentage of tetraploids was20% in total treated bulbs. Treatment at higher concentration and longer duration increased the percentage of dead plants up to 100%. Thirty six tetraploid plants of shallot were produced.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.3.