Antibiotic Resistance Profile of Colistin-Resistant Escherichia coli Isolated from Raw Pork Sold at Retail Markets

Date Received: 18-06-2025

Date Accepted: 07-12-2025

Date Published: 24-12-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2025.23.12.03

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Issue: Vol. 23 No. 12 (2025): Tạp chí Khoa học Nông nghiệp Việt Nam

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Ha, C., Duc, H., & Son, H. (2025). Antibiotic Resistance Profile of Colistin-Resistant Escherichia coli Isolated from Raw Pork Sold at Retail Markets. Vietnam Journal of Agricultural Sciences, 23(12), 1582–1591. https://doi.org/10.31817/tckhnnvn.2025.23.12.03

Antibiotic Resistance Profile of Colistin-Resistant Escherichia coli Isolated from Raw Pork Sold at Retail Markets

Cam Thi Thu Ha (*) , Hoang Minh Duc 1 , Hoang Minh Son 1

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Escherichia coli, antibiotic resistance, colistin, raw pork

    Abstract


    The emergence and widespread dissemination of colistin-resistant E. coli is a significant challenge to both human and animal health. This study aimed to determine the prevalence and antibiotic resistance profile of colistin-resistant E. coli isolated from raw pork sold at traditional markets in Dong Anh, Hanoi. E. coli was isolated using culture on selective media. The antibiotic resistance of E. coli was determined using a microbroth dilution method following the guidelines of the Clinical and Laboratory Standards Institute. The mcr gene was detected by multiplex-PCR. The results of this study showed that 26% (13/50) of the pork samples were positive for colistin-resistant E. coli. High resistance levels were observed for florfenicol, ampicillin, streptomycin, tetracycline, trimethoprim/ sulfamethoxazole, and nalidixic acid, with resistance rates ranging from 84.62% to 100%. All colistin-resistant E. coli isolates were identified as multidrug-resistant strains and carried the mcr-1 gene. These findings highlight that raw pork poses a potential risk for the transmission of colistin-resistant E. coli from meat to humans, thereby increasing the risk of antimicrobial-resistant bacterial infections in the community.

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