In VitroHaploid Production by Anther Culture of Dong Du Mustard (Brassica juncea)

Date Received: 13-10-2014

Date Accepted: 22-07-2015

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Issue: Vol. 13 No. 5 (2015)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Thanh Hải N., & Tam, D. (2024). In VitroHaploid Production by Anther Culture of Dong Du Mustard (Brassica juncea). Vietnam Journal of Agricultural Sciences, 13(5), 739–746. https://vie.vjas.vn/index.php/vjasvn/article/view/218

In VitroHaploid Production by Anther Culture of Dong Du Mustard (Brassica juncea)

Nguyẽn Thanh Hải 1, 2, 3 , Dang Thi Thanh Tam 4

  • 1 Viện Sinh học Nông nghiệp, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Công nghệ Sinh học, Học viện Nông nghiệp Việt Nam
  • 4 Khoa Công nghệ Sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Androgenesis, anther microspore culture, Brassica juncea, Dong du mustard, embryogenesis, haploid line

    Abstract


    Androgenesis is a phenomenon in which microspores are made to bypass the sexual pathway and follow the sporophytic mode of development to generate new plants without the intervention of fertilization under in vitro conditions. Micorospore culture provides an ideal system, with a large, relatively uniform population of haploid cells, for use mutant selection, genetic transformation and in studies on the molecular mechanism of induction of androgenesis and embryogenesis. This paper aimed at establishing a protocol for anther microspore embryogenensis in traditional variety of Brassica juncea (Dong Du mustard). Five haploid lines was created from anther microspore culture. The results showed that, medium and plant growth regulator concentration had a pronounced effect on androgenic response in anther microspore cultures of Brassica juncea (Dong Du mustard). The medium B5 is the most suitable for morphogenesis of anther microspores culture. The highest percent of embryogenensis (2,33%) was obtained B5 medium supplemented with α-NAA 0,5 mg/l and Kinetin 1 mg/l. MS medium supplemented with IAA 0,5 mg/l, BA 0,5 mg/ l, 3% sugar and 8 g / l agar was capable of regenerating seedling from embryos. MS medium supplemented with 1,0 mg/l BA was optimal for shoot multiplication with a rate of 3.94 after four weeks. MS medium with 0,5 mg/l α-NAA was appropriate for root induction in terms of root number per shoot (6.54 root/shoot) and average length of root (3.68 cm) after three weeks.

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