Antibacterial Activity of Protein Hydrolysate from Black Soldier Fly (Hermetia illucens) Larvae Against Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Leg Shrimp (Litopenaeus vannamei)

Date Received: 05-09-2025

Date Accepted: 02-12-2025

Date Published: 28-03-2026

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Nhu, N., Duyen, T., Thy, H., & Quy, O. (2026). Antibacterial Activity of Protein Hydrolysate from Black Soldier Fly (Hermetia illucens) Larvae Against Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Leg Shrimp (Litopenaeus vannamei). Vietnam Journal of Agricultural Sciences, 24(3), 291–298. https://doi.org/10.31817/tckhnnvn.2026.24.3.01

Antibacterial Activity of Protein Hydrolysate from Black Soldier Fly (Hermetia illucens) Larvae Against Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Leg Shrimp (Litopenaeus vannamei)

Nguyen Lam Kim Nhu 1 , Tran Thi My Duyen 1 , Ho Thi Truong Thy (*) , Ong Moc Quy 2

  • Tác giả liên hệ: [email protected]
  • 1 Đại học Nông Lâm TPHCM
  • 2 KHOA THUỶ SẢN, ĐẠI HỌC NÔNG LÂM TPHCM
  • Keywords

    Acute hepatopancreatic necrosis disease, antimicrobial activity, black soldier fly, Hermetia illucens, white leg shrimp, Vibrio parahaemolyticus

    Abstract


    The study was conducted to investigate the antimicrobial activity of the black soldier fly (Hermetia illucens) larva protein hydrolysates on Vibrio parahaemolyticus bacteria causing acute hepatopancreatic necrosis in white leg shrimp. The study was conducted using well diffusion and serial dilution methods that determined the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of protein hydrolysates from black soldier fly larvae. In the diffuse well agar method, the results showed that at concentrations of 150 and 200 mg/ml, clear zone diameters were recorded at 13.7 ± 1.2 and 14.7 ± 0.6mm, respectively, and statistically significant different compared to negative controls. MIC values and MBC values were 50 mg/ml. The results noted that hydrolyzed protein from black soldier fly larvae was able to inhibit and kill Vibrio parahaemolyticus bacteria that cause acute hepatopancreatic necrosis disease in shrimp.

    Author Biographies

    Nguyen Lam Kim Nhu, Đại học Nông Lâm TPHCM

    Sinh viên

    Tran Thi My Duyen, Đại học Nông Lâm TPHCM

    Sinh viên

    Ong Moc Quy, KHOA THUỶ SẢN, ĐẠI HỌC NÔNG LÂM TPHCM

    Giảng Viên

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