Date Received: 18-04-2017 / Date Accepted: 12-02-2018
To develop immunochromatographic strips for direct detection of the Streptococcus suistype 2 (S. suis2) antigen,1,5x108CFU of inactivated S. suis2 mixed well with Freund’s adjuvant was injected to Swiss albino mice to produce polyclonal antibodies. Then, the S. suis 2 polyclonal antibody (360 µg/ml) was mixed with gold colloid solution (pH 8.0) to create colloidal gold probe. Results indicated that the optimal concentration of capture antibodies in test lane was 2 mg/ml. The Kit had high sensitivity and specificity and no cross-reaction with E. coli ATCC,P. aeruginosaATCC, S. aureus ATCCor other strains ofS. suis. The detection sensitivity was found to be as high as 5x105CFU. In conclusion, the Kit is easy for the use and highlyefficient forS. Suistype 2 detection.