Characteristics of Colistin and Other Important Antibiotics Resistance Genes of Escherichia coli Isolated from Pig and Farm-Workers in Soc Son, Ha Noi

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Date Received: 26-02-2025

Date Published: 26-02-2025

##submissions.doi##: https://doi.org/10.1234/z77jv460

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Issue: Vol. 22 No. 11 (2024)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

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Son, D. T. T., Nhat, T. T., Thao, P. T. P., Thuy, N. T. T., Etiene, L., & My, T. N. (2025). Characteristics of Colistin and Other Important Antibiotics Resistance Genes of Escherichia coli Isolated from Pig and Farm-Workers in Soc Son, Ha Noi. Vietnam Journal of Agricultural Sciences, 22(11). https://doi.org/10.1234/z77jv460

Characteristics of Colistin and Other Important Antibiotics Resistance Genes of Escherichia coli Isolated from Pig and Farm-Workers in Soc Son, Ha Noi

Dang Thi Thanh Son (*) , Tran Thi Nhat , Phan Thi Phuong Thao , Nguyen Thi Thanh Thuy , Etiene Loire , Truong Nhat My

  • Tác giả liên hệ: thanhsonnivr@gmail.com

    • Keywords

    E. coli, antibiotic resistance, colistin, resistant-genes, pig

    Abstract


    Antibiotic-resistant bacteria and the circulation of drug-resistant genes are a threat to public health globally. Research results show that the rate of detected of colistin ressitant E. coli from pigs in small- scale pig farm in Soc Son - Hanoi was 72% (36/50 farms) and from farm-workers was 16% (8/50 farms). Of these, 10 E. coli strains from 05 farms were selected for analyzing their genomic sequences by Oxford Nanopore Technologies (ONT) systerm (one strain from pig and one strain from farm-worker each of five farms). The studied results showed that 38 resistance genes (14 genes located on chromosome và 31 genes located on plasmids) with 9 antibiotic group. 10 strains were determined to carry to 7-22 resistance genes simultaneously. E. coli strains carried resistance genes of β-lactams, colistin, tetracycline, sulfonamide, quinolon, phenicol, aminoglycoside, marcolide, trimethoprim. The mcr-1 and/or mcr-3 were found in 10 E. coli strains carrying colistin-resistance phenotype. One strain isolated from farmer and 3 strains isolated from pigs carried 2 mcr-1 and mcr-3 simultaneously. One E. coli strain (barcode 60 - PCE 20.1) was detected from pigs and another (barcode 67- PCE 31.1) was isolated from farm-workers were of the same resistance gen characteristic eventhought they were detected from two diffirent farms.

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