Date Received: 30-07-2014
Date Accepted: 14-10-2014
Date Published: 06-08-2025
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.
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Effect of Light Sources and Containers on Rapid Multiplication and Growth of Dianthus Variety Hong Hac in Tissue Culture
Keywords
Carnation, Hong Hac, LED lamp, multiplication
Abstract
Effect of light sources and containers on rapid multiplication and growth of Dianthus variety Hong Hac in tissue culture were studied. The results showed that, the use of cylinder flask closed with a cotton plug and plastic bag ventilated with a filter membrane gave highest plant quality. In rapid multiplication phase, lamp type LED 17R-3B had highest effects on formation of leaf, shoots and plant quality when cultured in cylinder flask closed with both cotton plug and/or plastic bag ventilated with filter membrane. LED 13R-4B-3W had positive effects on plant quality compared to other lamp types.
References
Gamborg OL, Phillips GC (1995). Plant cell, tissue and organ culture: Fundamental methods. Springer-Verlag Berlin Heidelberg GmbH, pp. 358
Jackson MB, Abbott AJ, Belcher AR, Hall KC, Butler R, Cameron J (1991). Ventilation in plant tissue cultures and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development. Annals of Botany, 67: 229-237
Jain A, Husain S, Kothari SL (1997). Micropropagation of Dianthus caryophyllus L. - Control of vitrification. Journal of Plant Biochemistry and Biotechnology, 6: 35-37
Jordan KA, Norikane J, Takakura T (2001). Control of LED to achieve light quality and intensity in tissue culture and micro-propagation studies. Acta Hort., 562: 135-140
Kane ME, Philman NL (1992). Effect of culture vessel type on in vitro multiplication of Pontederia cordata L. Proc. Fla. State Hort. Soc., 105: 213-215
Kharrazi M, Nemati H, Tehranifar A, Bagheri A, Sharifi A (2011). In vitro culture of Carnation (Dianthus caryophyllus L.) focusing on the problem of vitrification. J. BIOL. ENVIRON. SCI., 5(13): 1-6
Pasqualetto PL (1990). Vitrification in plant tissue culture. In R Rodríguez, RS Tamés, DJ Durzan, eds, Plant Aging, Springer US, 186: 133-137
Rossetto M, Dixon K, Bunn E (1992). Aeration: A simple method to control vitrification and improve in vitro culture of rare australian plants. In Vitro - Plant, 28: 192-196
Sato S, Hagimori M, Iwai S (1993). Recovering vitrified carnation (Dianthus caryophyllus L.) shoots using Bacto-Peptone and its subfractions. Plant Cell Reports, 12: 370-374
Stutte WG (2009). Light-emitting diodes for manipulating the phytochrome apparatus. Hortscience, 44: 231-234
Yanagi T, Yachi T, Okuda N, Okamoto K (2006). Light quality of continuous illuminating at night to induce floral initiation of Fragaria chiloensis L. CHI-24-1. Scientia Horticulturae, 109: 309-314
Yano A, Fujiwara K (2012). Plant lighting system with five wavelength-band light-emitting diodes providing photon flux density and mixing ratio control. Plant Methods, 8: 1-12