Date Received: 25-07-2014
Date Accepted: 14-10-2014
Date Published: 06-08-2025
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.
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How to Cite:
Morphogenesis of Calli and Shoots of Cinnamomum camphora (L.) Sieb.
,
Tran Van Tien
,
Vo Thi Bach Mai
,
Tran Trong Tuan
,
Nguyen Hai Son (*)
Keywords
Cinnamomum camphora (L.) Sieb., callus, morphogenesis, WPM medium, shoot
Abstract
The organogenesis (shoots and roots) is a complicated process, influenced by various endogenous and exogenous factors. Therefore, to understand the formation of the initials, several techniques have been used as anatomy, inhibitors and plant growth regulators supplement. In this study, we investigated the effects of plant growth regulators on the callogenesis, shoot formation and the process of shoot morphogenesis of Cinnamomum camphora (L.) Sieb. The results showed that the WPM medium supplemented with 2% sucrose, 0.5 mg/l NAA and 0.2 mg/l BA was suitable for the callus formation. WPM medium supplemented with 2% sucrose and 1 mg/l BA was the excellent medium for shoot multiplication (the mean of 7.7 buds/explant and 5.57g fresh weight). Morphological anatomy of shoots showed that the cortex parenchyma cell is the position to initiate shoot formation.
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