Date Received: 02-03-2021
Date Accepted: 19-07-2021
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In Vitro Micropropagation of Strawberry CV. Sunraku Introduced from Japan
,
Tran Thi My Duyen
2
,
Do Thi Mai
1
,
Nguyẽn Thanh Hải
1, 3, 4
Keywords
Shromosome, micropropagation, runner, strawberry, cv. Sunraku
Abstract
With the aim of creating disease-free, homogeneous seedlings for growers in large quantity in a short time, this study evaluates the effect of the HgCl2(mercury chloride), BA (N6-benzyladenine), Pic (picloram), TDZ (thidiazuron), kinetin, IBA (indole-3-butanoic acid), and α-NAA (α-naphthaleneacetic acid) at different concentrations on in vitro propagation of strawberry cv. Sunraku. Research results showed that using 0.1% HgCl2for five minutes gave 100% disease-free samples on the MS medium. MS medium supplemented with 0.75 mg/l BA and 0.3 mg/l IBA gave the highest shoot number (23.1 shoots per explant) and good quality. Half MS medium combined with 0.3 mg/l α-NAA was the best medium for rooting (18.6 roots per shoot). The research also showed that in vitroseedlings have the highest surviral rate (100%) and best development when they were grown on a mixed substrate (1 soil : 2 burn rice husks : 1 coconut fiber). The number of chromosomes (2n = 8x = 56) of regenerated plants was similar to that of the mother plant.
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