Date Received: 07-06-2013 / Date Accepted: 18-09-2013 / Date Published: 13-06-2025
Protocol for in vitro multiplication of Philodendrom xanadu was established including following steps: initial culture, shoot propagation and rooting of in vitro shoots and in vitro plantlet climatization. To create initial materials, apical buds were cultured on MS medium containing benzyl adenine (BA) or kinetin (KI). The results showed that MS medium supplemented with 4.0 mg/lmg/l BA was optimal for shoot multiplication The effect of the combination of cytokinin (BA) and auxin (IAA, IBA) on shoot multiplication of Philodendrom xanadu was also investigated. The results showed that addition of IAA or IBA on basal medium (MS medium containing 4.0 mg/l BA) did not increase multiplication rate. α-NAA or activated charcoal had positive impact on in vitro shoot rooting. 1g/l activated charcoal-added MS medium was appropriate for root induction in terms of root number per shoot and average length of root after four weeks culture. After in vitro multiplication step, in vitro plantlets were transferred to greenhouse onto four different substrates. Survival percentage of plantlets was 100% on all substrates and acclimatized plantlets showed the best growth and development on mixture of coconut fiber and burnt rice husk (1:1).
