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• Optimizaton and Evaluation of Multiplex PCR forAnalysis of Pompano(Trachinotusspp.)

  Luu Thi Ha Giang, Dang Thi Nguyen, Tran Thi Thuy Ha, Phan Thi Van

  Date Received: 04-04-2018 / Date Accepted: 08-05-2018

   Abstract PDF (VIETNAMESE)

  In this study, fifteen microsatellite markers were used to optimize a protocol for analyzing genetic diversityof pompano (Trachinotusspp.). Seven single markers were successfully optimized for short fin Pompano and four markers were optimized for long fin pompano. Three multiplex PCR reactionsincludingtwo multiplex pools for short fin Pompano and one pool for long fin pompano were developed which composed of nine fluorescent-labeled markers. Fragment analysis process was successfully established with three pools of microsatellite markerscorresponding to nine loci. Genotyping results revealedpolymorphism in eight loci (EC-7; EC-9; EC-10; EC-17; EC-20; EC-28; TB018; TBG030) with the number of alleles rangingfrom 3 to 6, whereas locus TO67 showed monomorphisms. Thesefindingsimportantlycontributeto the understanding of genetic diversity in pompano.

• Apparent Digestibility of Some Commercial Feeds for Short Fin Pompano (Trachinotus ovatus)

  Tran Thi Nang Thu, Nguyen Huu Ninh, Tran The Muu, TranThi Thap Hieu, Nguyen Tuan Dat

  Date Received: 20-10-2016 / Date Accepted: 17-07-2016 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  The study was carried out from March to June 2015 at National Broodstock Center for Mariculture Species in Northern Vietnam (Center), Cat Ba, Hai Phong province, to evaluate digestibility of protein (ADP), lipid (ADL) and gross energy (ADE) of some available commercial feeds for pompano,Trachinotus ovatus, using indirect method with chromic oxidize (Cr2O3) 1% as inert marker. Pompano of 400g/fish were held in marine cage- culture system (3 x 3 x 3 m, six of each cage) at density of 10fish/m³. Three commercial aquafeed brands with different protein content,46, 50 and 51% were determined for their digestibility inpompano (abbreviated as D46, D50 and D51) with three replicates. The feaces were collected each day by stripping method before feeding the fish. Fish were fed by hand to apparent satiety, once daily at 9h. Feaces werethen pooled in sealed bottles and stored at- 200C until use. The results showed that ADP of D50 (71.34%) was lowest among experimental diets (P< 0.05), while that of D46 (76.42%) and D51 (77.52%) did not significantly differ (P> 0.05). ADL value of D51 was lower than that of D46 and D50 (P< 0.05). Result of ADE indicated that ability of pompano to digest energy from three commercial diets was similar (P> 0.05). The digestibility of protein and energy of three pellet feedsusedto test in pompano culture wasnot so highandthe quality of these feedsshould be further improved.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.8.

• Effects of Temperature on Embryonic Development and MalflormationEof Pompano,Trahinotus blochii

  Tran Thi Mai Huong, Nguyen Thi Nien, Dam Thi My Chinh, Le Van Khoi, Nguyen Huu Ninh

  Date Received: 27-07-2016 / Date Accepted: 28-12-2016 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The experiment of the effect of temperatureeffecton embryonic development and malformation of pompano (Trachinotus blochii) was carried out at the Northen National Broodstock Center for Marine aquaculture oin Cat Ba, Hai Phong, Vietnam. Five temperature levels of 24oC, 26oC, 28oC, 30oC and 32oC were studied with 3 replicates each. The results showed that,there was a significant differencetbetween five treatments in terms of hatching, deformity and survival rates. The result indicated that the significant highest hatching rate was 47.7% at 24oC. The lowest deformity rate was observed at 24oC (3.3%) but no significant difference was found between 24oC, 26oC and 28oC. The survival rate was higher at 26oC (58,6%) and 28oC (58,2%) after five days of post-hatching than other. The results show that sSuitable temperatures for pompano incubation are ranged from 24oC to 28oC. At 26oC and 28oC, the lowest deformity rate (3,98 - 4,20%) and the highest survival rate (10.48% and 10.69%) after 40 days of post hatching were recorded. From this study, the temperature rangedfrom 26 - 28oC are recommended for pompano rearing.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.12.

• Identification and Genetic Assessment of the Pompano Based on the Molecular Markers

  Tran Thi Thuy Ha, Luu Thi Ha Giang, Vu Thi Trang, Pham Hong Nhat,  Phan Thi Van

  Date Received: 05-04-2019 / Date Accepted: 14-06-2019

   Abstract PDF (VIETNAMESE)

  This study aimed to identify species andassessgenetic diversity of four pompano populations collected in Nha Trang, Vung Tau, Hai Phong and Quang Ninh. The molecular makers based on COI sequencing and microsatellite markers were applied. The results revealed that the sequences of the COI gene isolated form Vietnamese pompano were highly similar (99-100%) to the COI sequences of pompano Trachinotus ovatus(Genbank accession number: KF356397.1, HQ127346.1 and 10 KJ642220.1). Genetic diversity inferred from microsatellite markers indicated high allele polymorphism (average of 8-15.33 alleles) and high polymorphism information content (average of PIC values: 0.685-0.839). The coefficient of inbreeding Fis>0 was recorded in Hai Phong and Vung Tau populations, while Nha Trang and Quang Ninh fish populations contained low inbreeding coefficient with Fis <0. The genetic relationship reflected by Fst coefficient indicated the moderated level of genetic difference amongstfour populations, in which the Vung Tau population is more closely related to the populations of Hai Phong, Quang Ninh and Nha Trang. The analysis revealed an unclearpopulation structure according tothe Analysis of Molecular Variance (AMOVA)results. This study mightsupport for managing the stockof selective breeding program of the pompano.

• Overview of Diseases Caused By Streptococcus iniae in Marine Fish

  Nguyen Cong Thiet, Kim Minh Anh, Doan Thi Nhinh, Truong Dinh Hoai

  Date Received: 12-05-2025 / Date Accepted: 14-08-2025 / Date Published: 28-08-2025

   Abstract PDF (VIETNAMESE)

  Streptococcus iniae is a highly pathogenic microorganism that poses significant economic threats to marine aquaculture, notably impacting commercially valuable species such as snubnose pompano, seabass, groupers, and snappers. The recent emergence of new infection cases in both farmed and wild fish populations, domestically and internationally, underscores the urgent necessity for comprehensive data collection for the development of effective disease management strategies. Such measures are vital to reducing economic losses in Vietnam’s marine fisheries sector. This review explores the mechanisms of infection, virulence factors, antibiotic resistance, and the current approaches to disease control in marine fish affected by S. iniae. The review highlights ongoing research and practical applications involving vaccines, probiotics, and botanical products aimed at enhancing immunological defences and resistance against this pathogen. Furthermore, the review discusses future prospects and research directions pertinent to S. iniae infections in Vietnamese marine fish, thereby supporting the advancement of sustainable aquaculture practices and effective disease management.

  DOI: https://doi.org/10.31817/tckhnnvn.2025.23.8.14

• Influence of Environmental Factors on the Growth of Streptococcus iniae Isolated from Diseased Pompano (Trachinotus spp.)

  Nguyen Cong Thiet, Truong Anh Vu, Le Thi Lan Huong, Doan Thi Nhinh, Kim Minh Anh, Dang Thi Hoa, Nguyen Anh Tuan, Truong Dinh Hoai

  Date Received: 16-07-2025 / Date Accepted: 17-09-2025 / Date Published: 31-10-2025

   Abstract PDF (VIETNAMESE)

  This study aimed to evaluate the impact of key environmental factors on the growth of Streptococcus iniae, a pathogenic bacterium affecting Pompano (Trachinotus spp.), under in vitro conditions. Fish samples (n = 54) exhibiting exophthalmia and hemorrhages, collected from Quang Ninh, Hai Phong, and Ninh Binh, were used for bacterial isolation. Bacterial identification was performed through morphological analysis, biochemical characterization, PCR techniques, and experimental infection. A representative S. iniae isolates from each province was utilized to assess the effects of temperature, pH, and salinity on bacterial growth. A total of nine S. iniae strains were successfully isolated and identified. The evaluation of environmental influences after 36 hours of cultivation on three representative isolates revealed that S. iniae can grow within a temperature range of 15-45°C, with optimal growth observed between 25-40°C. The bacteria grow well at pH of 6-8, with optimal at neutral pH (pH = 7). Additionally, S. iniae can survive and develop at salinities ranging from 0 to 60‰. However, its growth capacity tends to decline gradually with increasing salinity. The findings provide a scientific basis for developing strategies to prevent S. iniae-related diseases in pompano fish.

  DOI: https://doi.org/10.31817/tckhnnvn.2025.23.10.04