Investigating OsHSBP1 Gene Expression in Relation to Drought and Heat Responses in Rice (Oryza sativa L.)

Date Received: 08-02-2025

Date Published: 21-02-2025

##submissions.doi##: https://doi.org/10.1234/jq9q4727

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Issue: Vol. 22 No. 11 (2024)

Section:

NÔNG HỌC

How to Cite:

Quyen, C., Huong, P., Hieu, D., Hoi, P., & Phuong, N. (2025). Investigating OsHSBP1 Gene Expression in Relation to Drought and Heat Responses in Rice (Oryza sativa L.). Vietnam Journal of Agricultural Sciences, 22(11). https://doi.org/10.1234/jq9q4727

Investigating OsHSBP1 Gene Expression in Relation to Drought and Heat Responses in Rice (Oryza sativa L.)

Cao Le Quyen , Phung Thi Thu Huong , Dam Quang Hieu , Pham Xuan Hoi , Nguyen Duy Phuong (*)

  • Tác giả liên hệ: phuongnd.bio@gmail.com

    • Keywords

    Gene expression, drought stress, heat stress, OsHSBP1

    Abstract


    The production of rice is significantly affected by adverse environmental conditions such as drought and heat. Heat shock proteins (HSPs) play a crucial role in mitigating stress tolerance responses associated with temperature and oxidation, thereby safeguarding cells and macromolecules from detrimental effects. The regulatory mechanism of HSP activity in some plant species involves the involvement of Heat Shock Factor Binding Protein (HSBP). This study aimed to investigate the functional activities and elucidate the stress response regulation mechanism of OsHSBP1 in rice. The expression pattern of this gene was analyzed under drought and heat stress conditions in two rice varieties, BC15 and OM54541. The OsHSBP1 expression increased at various time points during artificial heat and drought stress. The coding sequences of OsHSBP1 from these rice varieties were isolated and fully sequenced, revealing a gene segment of 237 base pairs in length. This segment encodes a polypeptide chain comprising 78 amino acids, including an α helix, exhibiting a similarity ranging from 59.8% to 97.4% homologous to HSBP proteins studied previously. These findings provide a foundation for exploring the functional role of OsHSBP1 to improve the drought and heat stress resistance of the major rice varieties such as BC15 and OM5451 through genetic engineering..

    References

    Cardi T., Murovec J., Bakhsh A., Boniecka J., Bruegmann T., Bull S.E., Eeckhaut T., Fladung M., Galovic V., Linkiewicz A., Lukan T., Mafra I., Michalski K., Kavas M., Nicolia A., Nowakowska J., Sági L., Sarmiento C., Yýldýrým K., Zlatković M., Hensel G., Van Laere K. (2023). CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation. Trends Plant Science. 28(10): 1144-1165.

    Firmansyah & Argosubekti N. (2020). A review of heat stress signaling in plants”. IOP Conference Series: Earth and Environmental Science. 484(1): 012041.

    Fu S., Meeley R. & Scanlon M.J. (2002). Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis. The Plant Cell. 14: 3119-3132.

    Gu L., Jiang T., Zhang C., Li X., Wang C., Zhang Y., Li T., Dirk L.M.A., Downie A.B., Zhao T. (2019). Maize HSFA2 and HSBP2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis. Plant J. 100(1): 128-142.

    Hsu S.F., Lai H.C. & Jinn T.L. (2010). Cytosol-localized heat shock factor binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis. Plant Physiology. 153: 773–784.

    Hu C., Yang J., Qi Z., Wu H., Wang B., Zou F., Mei H., Liu J., Wang W., Liu Q. (2022). Heat shock proteins: Biological functions, pathological roles, and therapeutic opportunities. MedComm.

    (3): e161.

    Jain M., Nijhawan A., Tyagi A.K. & Khurana J.P. (2006). Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochemical and Biophysical Research and Communications. 345(2): 646-651.

    Jewell M.C., Campbell B.C & Godwin I.D. (2010). Transgenic plants for abiotic stress resistance. Transgenic Crop Plants, Springer, Berlin. 67-132.

    Li T., Zhang Y., Liu Y., Li X., Hao G., Han Q., Dirk L.M.A., Downie A.B., Ruan Y.L., Wang J., Wang G. & Zhao T. (2020). Raffinose synthase enhances drought tolerance through raffinose synthesis or galactinol hydrolysis in maize and Arabidopsis plants. Journal of Biological Chemistry.

    (23): 8064-8077.

    Mohamed H. & Al-Whaibi (2011). Plant heat-shock proteins: A mini review. Journal of King Saud University - Science. 23(2): 139-150.

    Morimoto R.I. & Santoro M.G. (1998). Stress-inducible responses and heat shock proteins: new pharmacologic targets for cytoprotection. Nature Biotechnology. 16(9): 833-8.

    Muthusamy M., Son S., Park S.R. & Lee S.I. (2023). Heat shock factor binding protein BrHSBP1 regulates seed and pod development in Brassica rapa. Frontier in Plant Science. 14:1232736.

    doi: 10.3389/fpls.2023.1232736

    Qin Q.L., Liu J.G., Zhang Z., Peng R.H., Xiong A.S., Yao Q.H. & Chen J.M. (2007). Isolation, optimization, and functional analysis of the cDNA encoding transcription factor RDREB1 in Oryza sativa L.”, Mol. Breed. 19(4): 329-340.

    Qu A.L., Ding Y.F., Jiang Q. & Zhu C. (2013). Molecular mechanisms of the plant heat stress response. Biochem. Biophys. Research

    Communication. 432(2): 203-207.

    Rana R.M., Dong S., Tang H., Ahmad F. & Zhang H. (2012). Functional analysis of OsHSBP1 and OsHSBP2 revealed their involvement in the heat shock response in rice (Oryza sativa L.). Journal of Experimental Botany. 63(16): 6003-6016.

    Razzaq A., Saleem F., Kanwa, M., Mustafa G., Yousaf S., Imran Arshad H.M., Hameed M.K., Khan M.S. & Joyia F.A. (2019). Modern trends in plant genome editing: an inclusive review of the crispr/cas9 toolbox. International Journal of Molecular Sciences. 20(16): 4045.

    Satyal S.H., Chen D., Fox S.G., Kramer J.M. & Morimoto R.I. (1998) . Negative regulation of the heat shock transcriptional response by HSBP1. Genes and Development. 12: 1962–1974.

    Waterhouse A., Bertoni M., Bienert S., Studer G., Tauriello G., Gumienny R., Heer F.T., de Beer T.A.P., Rempfer C., Bordoli L., Lepore R. & Schwede T. (2018). SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Research. 46(W1): W296-W303.