Antimicrobial Property of Moringa Oleifera Leaves, Pods and Seeds Against Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveyi Causing Diseases in White Leg Shrimp (Penaeus vannamei)

Date Received: 27-12-2024

Date Accepted: 30-05-2025

Date Published: 30-05-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2025.23.5.

Views

39

Downloads

15

Issue: Vol. 23 No. 5 (2025): Tạp chí Khoa học Nông nghiệp Việt Nam

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hoài, T., Khiem, V., Anh, N., Hoa, D., Trinh, T., Nhinh, D., & Tuan, N. (2025). Antimicrobial Property of Moringa Oleifera Leaves, Pods and Seeds Against Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveyi Causing Diseases in White Leg Shrimp (Penaeus vannamei). Vietnam Journal of Agricultural Sciences, 23(5), 586–597. https://doi.org/10.31817/tckhnnvn.2025.23.5.

Antimicrobial Property of Moringa Oleifera Leaves, Pods and Seeds Against Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveyi Causing Diseases in White Leg Shrimp (Penaeus vannamei)

Trương Đình Hoài (*) , Vuong Huy Khiem 1 , Nguyen Thao Anh 1 , Dang Thi Hoa 1 , Tran Thi Trinh 1 , Doan Thi Nhinh 1 , Nguyen Ngoc Tuan 1

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Thuỷ sản, Học viện Nông nghiệp Việt Nam

    • Keywords

    Moringa oleifera extract, Antibacterial, Whiteleg shrimp, Vibrio spp.

    Abstract


    This study evaluated the antibacterial activity of extracts from different parts of the moringa plant against pathogenic bacteria in whiteleg shrimp. A total of 9 bacterial strains were successfully isolated and identified, including Vibrio parahaemolyticus (n = 3) from shrimp infected with acute hepatopancreatic necrosis, V. alginolyticus (n = 3) from shrimp infected with white feces syndrome, and V. harveyi (n = 3) from luminous infected shrimp. From 500g of each part, 278ml of immature fruit extract, 170ml of fresh leaf extract, and 250ml of seed oil were obtained. The results from the inhibition assessment using the disk diffusion method showed that the inhibition zones for the three bacterial species ranged from 12.8 to 13.4mm for leaf extracts and 14.7 to 15.7mm for immature fruit extracts, while the seed oil did not produce any inhibition zones. The fresh leaf extract completely inhibited (MBC)
    V. parahaemolyticus at a dilution ratio of 1:16 (3.13% extract) and for the two species V. alginolyticus and V. harveyi at 1:64 (0.78% extract). The immature fruit extract achieved an MBC value of 1:32 (1.56% extract) against
    V. parahaemolyticus and 1:128 (0.39% extract) against the other two bacterial species. The study results demonstrate the potential application of fresh extracts from moringa leaves and pods for controlling bacterial infections in shrimp..

    References

    Abidin Z., Huang H.T., Liao Z.H., Chen B.Y., Wu Y.S., Lin Y.J. & Nan F.H. (2021). Moringa oleifera leaves’ extract enhances nonspecific immune responses, resistance against Vibrio alginolyticus, and growth in whiteleg shrimp (Penaeus vannamei). Animals. 12(1): 42.

    Aminzare M., Hashemi M., Abbasi Z., Mohseni M. & Amiri E. (2018). Vibriosis phytotherapy: A review on the most important world medicinal plants effective on Vibrio spp. Journal of Applied Pharmaceutical Science. 8(01): 170-177.

    Bancessi A., Pinto M.M.F., Duarte E., Catarino L. & Nazareth T. (2020). The antimicrobial properties of Moringa oleifera Lam. for water treatment: a systematic review. SN Applied Sciences. 2: 1-9.

    Chellapandian H., Sivakamavalli J., Anand A.V. & Balasubramanian B. (2021). Challenges in controlling vibriosis in shrimp farms. Infections and sepsis development. 195.

    Conejero M. & Hedreyda C. (2003). Isolation of partial toxR gene of Vibrio harveyi and design of toxR‐targeted PCR primers for species detection. Journal of Applied Microbiology. 95(3): 602-611.

    Faikoh E.N., Hong Y.-H. & Hu S.-Y. (2014). Liposome-encapsulated cinnamaldehyde enhances zebrafish (Danio rerio) immunity and survival when challenged with Vibrio vulnificus and Streptococcus agalactiae. Fish & Shellfish Immunology. 38(1): 15-24.

    FAO (2020). The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome. https://doi.org/10.4060/ca9229en.

    Flores B., Ramírez E., Moncada A., Salinas N., Fischer R., Hernández C., Mora‐Sánchez B., Sheleby‐Elías J., Jirón W.& Balcázar J.L. (2022). Antimicrobial effect of Moringa oleifera seed powder against Vibrio cholerae isolated from the rearing water of shrimp (Penaeus vannamei) postlarvae. Letters in Applied Microbiology. 74(2): 238-246.

    Hồng Mộng Huyền, Võ Tấn Huy & Trần Thị Tuyết Hoa (2018). Hoạt tính kháng khuẩn của một số cao chiết thảo dược kháng vi khuẩn gây bệnh ở tôm nuôi. 54(2): 143-150

    Kamel C. (2001). Natural plant extracts: Classical remedies bring modern animal production solutions. Cahiers options méditerranéennes. 54(3): 31-38.

    Kenconojati H., Ulkhaq M.F., Azhar M.H. & Rukmana N.R. (2023). Vibriocidal Activity of Ethanol Extract of Moringa Leaves and Its Effect on the Growth of Pacific White Shrimp. Jurnal Medik Veterinar. 6(1).

    Kumar R., Ng T.H. & Wang H.C. (2020). Acute hepatopancreatic necrosis disease in penaeid shrimp. Reviews in Aquaculture. 12(3): 1867-1880.

    Kumar T.S., Makesh M., Alavandi S. & Vijayan K. (2022). Clinical manifestations of White feces syndrome (WFS): and its association with Enterocytozoon hepatopenaei in Penaeus vannamei grow-out farms: A pathobiological investigation. Aquaculture. 547: 737463.

    Kumara K.R.P.S., & Hettiarachchi M.H. (2017). White faeces syndrome caused by Vibrio alginolyticus and Vibrio fluvialis in shrimp, Penaeus monodon (Fabricius 1798) - multimodal strategy to control the syndrome in Sri Lankan grow-out ponds. Asian Fish Sci. 30(4): 245-261.

    Nguyễn Thị Hồng Nhi (2020). Hoạt tính kháng khuẩn của cao chiết chùm ngây kháng vi khuẩn Vibrio spp. gây bệnh trên tôm thẻ chân trắng trong điều kiện in vitro. Tạp chí Khoa học Công nghệ Nông nghiệp Việt Nam. 5(144): 77-81.

    Nguyễn Lĩnh Nhân & Nguyễn Thị Thu Hương (2015). Khảo sát hoạt tính chống oxy hóa và kháng viêm của cao chiết từ hạt chùm ngây (Moringa oleifera lamk.). Tạp chí Y học Thành phố Hồ Chí Minh. 9(15): 175-181.

    Nguyễn Ngọc Phước, Nguyễn Thị Xuân Hồng & Nguyễn Công Chung (2020). Nghiên cứu độc lực của một số chủng vi khuẩn Vibrio parahaemolyticus gây bệnh hoại tử gan tụy cấp (AHPND) trên tôm chân trắng (Litopenaeus vannamei) nuôi tại Thừa Thiên Huế. Tạp chí Khoa học Nông nghiệp Việt Nam. 18(3): 202-211

    Nguyễn Trọng Nghĩa, Đặng Thị Hoàng Oanh, Trương Quốc Phú & Phạm Anh Tuấn (2015). Phân lập và xác định khả năng gây hoại tử gan tụy của vi khuẩn Vibrio paraheamolyticus phân lập từ tôm nuôi ở Bạc Liêu. Tạp chí Khoa học Trường Đại học Cần Thơ. (39): 99-107.

    Nurhafizah W.W.I., Lee K.L., Nadirah M., Danish-Daniel M., Zainathan S.C. & Najiah M. (2021). Virulence properties and pathogenicity of multidrug-resistant Vibrio harveyi associated with luminescent vibriosis in Pacific white shrimp, Penaeus vannamei. Journal of Invertebrate Pathology. 186: 107594.

    Olise N.A., Urama E.U., Odeyemi O., Oshim I.O. & Obroh A.A. (2021). Demonstration of the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of both Moringa oleifera and Gongronema latifolium extracts mixture against Staphylococcus aureus, Salmonella typhi and Escherichia coli. Journal of Advances in Medical and Pharmaceutical Sciences. 23(1): 46-52.

    Pérez-Sánchez T., Mora-Sánchez B. & Balcázar J.L. (2018). Biological approaches for disease control in aquaculture: advantages, limitations and challenges. Trends in microbiology. 26(11): 896-903.

    Piamsomboon P. & Han J.E. (2022). White feces syndrome, a multifactorial syndrome of cultured shrimp: a mini review. Fishes. 7(6): 339.

    Pratiwi R.D., Kuswandari S. & Utomo R.B. (2023). Antibacterial effect of ethanol extract of moringa oleifera seeds against Enterococcus faecalis ATCC 29212. Odonto: Dental Journal. 10(2): 180-187.

    Suhartono S., Ismail Y.S., Muhayya S.R. & Husnah M. (2019). Ethanolic extracts of Moringa oleifera leaves inhibit biofilm formation of Vibrio alginolyticus in vitro. Earth and Environmental Science. 348(1): 012018.

    Theethakaew C., Nakamura S., Motooka D., Matsuda S., Kodama T., Chonsin K., Suthienkul O. & Iida T. (2017). Plasmid dynamics in Vibrio parahaemolyticus strains related to shrimp Acute Hepatopancreatic Necrosis Syndrome (AHPNS). Infection, Genetics and Evolution. 51: 211-218.

    Tinwongger S., Proespraiwong P., Thawonsuwan J., Sriwanayos P., Kongkumnerd J., Chaweepack T., Mavichak R., Unajak S., Nozaki R. & Kondo H. (2014). Development of PCR diagnosis for shrimp Acute Hepatopancreatic Necrosis Disease (AHPND) strain of Vibrio parahaemolyticus. Fish Pathology. 49(4): 159-164.

    Vinay T.N., Ray A.K., Avunje S., Thangaraj S.K., Krishnappa H., Viswanathan B., Reddy M.A., Vijayan K.K. & Patil P.K. (2019). Vibrio harveyi biofilm as immunostimulant candidate for high-health pacific white shrimp, Penaeus vannamei farming. Fish & Shellfish Immunology. 95: 498-505.

    Wang H., Wan X., Xie G., Dong X., Wang X. & Huang J. (2020). Insights into the histopathology and microbiome of Pacific white shrimp, Penaeus vannamei, suffering from white feces syndrome. Aquaculture. 527: 735447.

    Zhou S., Hou Z., Li N. & Qin Q. (2007). Development of a SYBR Green I real‐time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood. Journal of Applied Microbiology. 103(5): 1897-1906.