The Crosstalk Between nicotine and PACAP in the Appetite Regulation in the Mouse Hypothalamus

Date Received: 31-10-2022

Date Accepted: 27-01-2023

Date Published: 03-07-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2023.21.1.

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Issue: Vol. 21 No. 1 (2023)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Trung, N., Kambe, Y. ., Anh, T., & Cuong, N. (2025). The Crosstalk Between nicotine and PACAP in the Appetite Regulation in the Mouse Hypothalamus. Vietnam Journal of Agricultural Sciences, 21(1), 31–39. https://doi.org/10.31817/tckhnnvn.2023.21.1.

The Crosstalk Between nicotine and PACAP in the Appetite Regulation in the Mouse Hypothalamus

Nguyen Thanh Trung (*) , Yuki Kambe , Tran Thi Anh , Nguyen Manh Cuong

  • Tác giả liên hệ: [email protected]

    • Keywords

    Nicotine, food intake, body weight, PACAP, mouse

    Abstract


    Nicotine is one of the causes of suppressed appetite. Many reports have shown the underlying neurobiological mechanisms of the anorexia effects of cigarette smoking through nicotine receptors in the brain. However, whether nicotine may act through an additional pathway regulated by polypeptide pituitary adenylate cyclase (PACAP) in the brain is yet to be determined. To address these issues, we used pharmacological combination such as overexpression, knockdown PACAP gene in VMH and feeding in this study. At first, we found that dose-dependent nicotine decreased the food intake in mice treated daily for 5 days. In addition, more pronounced effects of inhibition feeding were observed in PACAP knockout mice. Furthermore, overexpression of PACAP in the VMH increased the expression while knockdown of PACAP in the VMH decreased the expression of α4 of Nicotine. Taken together, these results suggested that the expression of α4 receptor of Nicotine in the hypothalamus might be regulated by PACAP in the VMH.

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