Date Received: 15-09-2025
Date Accepted: 02-07-2026
Date Published: 27-08-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.8.03
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Developmental Characteristics and Pathogenicity of Vibrio Parahaemolyticus Strain Ced25.047 isolated from Post-Larvae of White-Leg Shrimp (Penaeus vannamei) Affected by Translucent Post-Larvae Disease
,
Nguyen Thi Hanh
1
,
Nguyen Minh Quan
1
,
Nguyen Thi Nguyen
1
,
Truong Thi My Hanh
1
,
Phan Trong Binh (*)
1
,
Dang Thi Lua
1
Keywords
Whiteleg shrimp, Penaeus vannamei, Translucent Post-larvae Disease, salinity, toxicity
Abstract
The study was conducted to determine the pathogenic characteristics of Vibrio parahaemolyticus causing Translucent Postlarvae Disease (TPD) in whiteleg shrimp (Penaeus vannamei) at the postlarval stage, and to evaluate the effect of salinity on bacterial growth. Shrimp samples were analyzed to identify the causative agent using both conventional methods and PCR technique. A total of six V. parahaemolyticus strains were isolated, of which two strains carried three virulence genes simultaneously and four strains carried a single gene. The V. parahaemolyticus strain carrying three TPD virulence genes, designated CED25.047, was tested at seven salinity levels (5, 10, 15, 20, 25, 30, and 35‰). Results showed that CED25.047 grew strongly at salinities ranging from 15 to 35‰, with optimal growth at 15-30‰, and was inhibited at 5‰ and 10‰. The median lethal dose (LD50) caused mortality at 18h and 40h with bacterial concentrations of 1.1 × 106 CFU/ml and 5.0 × 103 CFU/ml, respectively. Histopathological observations revealed typical lesions in the hepatopancreas, including necrosis and degeneration of hepatopancreatic tubule cells, epithelial cell sloughing, and nuclear shrinkage/deformation.
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