Date Received: 14-04-2025
Date Accepted: 21-01-2026
Date Published: 29-04-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.4.02
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Evaluating some Morphological Characteristics and Standardizing the Scientific Name for the Song Mai Tree Based on DNA Barcode
Keywords
DNA barcode, Japanese plum, Prunus salicina, psbA-trnH
Abstract
This study aimed to determine the scientific name of the song mai plant based on selected morphological characteristics and DNA barcoding. The song mai samples were evaluated morphologically in combination with DNA barcode sequence analysis of the psbA-trnH region. The morphological assessment showed that the collected song mai samples had a horizontally spreading growth habit, obovate leaves with acute leaf bases, round fruits with yellow flesh, and broadly elliptical seeds with high surface roughness. In addition, analysis of the psbA-trnH sequences indicated that the song mai samples shared up to 99.68% sequence similarity with Prunus salicina sequences in the NCBI GenBank database. Phylogenetic analysis further demonstrated that the song mai samples clustered with Prunus salicina with a high bootstrap value (73%). Therefore, both morphological characteristics and DNA barcoding of the psbA-trnH region support the conclusion that the scientific name of the song mai plant is Prunus salicina, belonging to the family Rosaceae.
References
Batnini M. A, Bourguiba H., Trifi-Farah N. & Krichen L. (2019). Molecular diversity and phylogeny of Tunisian Prunus armeniaca L. by evaluating three candidate barcodes of the chloroplast genome. Scientia Horticulturae. 245: 99-106. Dong W., Xu C., Li C., Sun J., Zuo Y., Shi S., Cheng T., Guo J. & Zhou S. (2015). ycf1. the most promising plastid DNA barcode of land plants. Scientific Reports. 5: 8348. Doyle J.J. & Doyle J.L. (1990). Isolation of Plant DNA from Fresh Tissue. Focus. 12(1): 13-15.
Đào Thị Thu Lành (2024). Thực trạng và đề xuất giải pháp phát triển cây mơ Hương Tích tại rừng đặc dụng Hương Sơn. Kỷ yếu Hội thào khoa học: Lịch sử trồng trọt, phát triển và những giá trị văn hóa của cây mơ Hương Tích, ngày 25/01/2024, Hà Nội. tr. 1-9. Głowacka A., Sitarek M., Rozpara E., & Podwyszyńska M. (2021). Pomological Characteristics and Ploidy Levels of Japanese Plum (Prunus salicina Lindl.) Cultivars Preserved in Poland. Plants (Basel). 10(5): 884. Halász J., Szendy G., Ivanovska B., Tóth E. G & Hegedűs A. (2023). The self-incompatibility locus and chloroplast DNA regions of Prunus domestica reflect the origin and genetic diversity of traditional cultivars. Journal of the American Society for Horticultural Science. 148(5): 230-239.
Le Thi Tuyet Cham, Vu Thi Mai Anh, Vu Thi Thuy Hang, Pham Thi Ngoc, Hoang Dang Dung, Doan Thu Thuy. (2025). Identification and genetic diversity of Japanese apricot (Prunus mume) accessions in northern Vietnam. Biodiversitas. 26: 2383-2392. Mehlenbacher S. A., Cociu V. & Hough F.L. (1991): APRICOTS (Prunus). In: James, N. Moore and James, R. Ballington Jr. (eds) Genetic resources of temperate fruit and nuts crop I. The International Society for Horticultural Science, Wageningen.Moore R, Yoneda K. 2022. 1029. Prunus mume: Rosaceae. Curtis's Bot Mag 39(3): 409-431. DOI: 10.1111/curt.12455.
National Center for Biotechnology Information (NCBI). (n.d.). (2024). BLAST® Help: Query input and data selection. Retrieved November 11, 2024, from https://blast.ncbi.nlm.nih.gov/Blast.cgi
Nguyễn Hữu Hải (2024). Sự đa dạng nguồn gen cây mơ tại Việt nam. Kỷ yếu Hội thảo khoa học: Lịch sử trồng trọt, phát triển và những giá trị văn hóa của cây mơ Hương Tích. Ngày 25/01/2024, Hà nội. tr. 20-24 Quan X., & Zhou S. (2011). Molecular identification of species in Prunus sect. Persica (Rosaceae), with emphasis on evaluation of candidate barcodes for plants. Journal of Systematics and Evolution, 49(2): 138-145.
Royal Botanic Gardens, Kew (2025). The World Checklist of Vascular Plants: Prunus salicina Lindl. Retrieved from https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:730232-1 on March 21, 2025. Sayed H.A., Mostafa S., Haggag I.M. & Hassan N.A. (2023). DNA Barcoding of Prunus Species Collection Conserved in the National Gene Bank of Egypt. Molecular Biotechnology. 65: 410-418. https://doi.org/10.1007/s12033-022-00530-z Tamura K., Stecher G. & Kumar S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution. 38(7): 3022-3027. doi:10.1093/molbev/msab120 Xue S., Shi T., Luo W., Ni X., Iqbal S., Ni Z., Huang X., Yao D., Shen Z. & Gao Z. (2019). Comparative analysis of the complete chloroplast genome among Prunus mume, P. armeniaca, and P. salicina. Horticulture Research. 6: 89. https://doi.org/10.1038/s41438-019-0171-1.