IN VITRO PROPAGATION OF COMMON BEAN (Phaseolus vulgaris L.)

Date Received: 07-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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NÔNG HỌC

How to Cite:

Thao, N., Thao, N., Hassan, F. ., & Jacobsen, H. . (2025). IN VITRO PROPAGATION OF COMMON BEAN (Phaseolus vulgaris L.). Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

IN VITRO PROPAGATION OF COMMON BEAN (Phaseolus vulgaris L.)

Ninh Thi Thao (*) , Nguyen Thi Phuong Thao , Fathi Hassan , Hans Jörg Jacobsen

  • Tác giả liên hệ: [email protected]

    • Keywords

    BAP, Phaseolus vulgaris L., tái sinh in vitro, α-NAA.

    Abstract


     The study was conducted to establish the protocol for in vitro regeneration of common bean (Phaseolus vulgaris L.). Cotyledonary nodes excised from 7-day in vitro seedlings were used as primary explants. The optimum treatment for shoot induction was MS salts and B5 vitamins (MSB5) medium supplemented with 11.1µM BAP and 0.5µM α-NAA, where 86.7% of cotyledonary nodes induced shoots with an average of 3.1 shoots/explant after 4 weeks of culture. Elongated, well developed individual shoots with 3-6 leaves were transferred to rooting medium containing 4.9µM of IBA. The average rooting frequency was 53.3% with a mean of 1.7 roots/shoot after 4 weeks of culture. 80% rooted plantlets survived during acclimatization period in greenhouse.

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