Establishing In Vitro Regeneration System for Rice

Date Received: 12-06-2014

Date Accepted: 20-09-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.8.

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Issue: Vol. 12 No. 8 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Huong, P., Thao, N., Linh, N., Hieu, N., & Thao, N. (2025). Establishing In Vitro Regeneration System for Rice. Vietnam Journal of Agricultural Sciences, 12(8), 1249–1257. https://doi.org/10.31817/tckhnnvn.2014.12.8.

Establishing In Vitro Regeneration System for Rice

Phan Thi Huong , Nguyen Thi Phuong Thao , Nguyen Thi Thuy Linh , Nguyen Trang Hieu , Ninh Thi Thao (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Rice, callus induction, shoot regeneration

    Abstract


    The study was carried out to examine callus induction and plant regeneration of seven rice varieties, BM9630, NV1, NV2, NV3, and J02 (japonica group) and Huong com and BC15 (indica group). Percentage of callus formation of all varieties ranged from 72 to 98%, especially, the callus induction of Huong com, NV1 and J02 was above 90% (97%, 92% and 98%, respectively). The optimum callus induction medium for Huong com variety and other japonica varieties was N6 + vitamin B5 + 3 mg/l 2,4-D + 100 mg/l myo-inositol + 500 mg/l L-proline + 500 mg/l L-glutamin + 300 mg/l casein hydrolysate + 30 g/l sucrose.  Temperature significantly affected callus formation and its quality; the highest percentage of callus induction was obtained at 280C. Shoot regeneration from callus of Huong com, BC15, and J02 varieties on four media were obtained, however,  BC15 showed poor regeneration capacity on all types of media. High shoot regeneration of J02 and Huong com was obtained on N6 + vitamin B5 + 3 mg/l BAP + 1 mg/l α-NAA, 100 mg/l myo-inositol, 500 mg/l L-proline, 500 mg/l L-glutamin, 300 mg/l casein hydrolysate, 30 g/l sucrose.

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