Vegetative Propagation of Phalaenopsis Sogo Yukidian

Date Received: 16-10-2014

Date Accepted: 24-11-2014

Date Published: 06-08-2025

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Son, N., Thach, N., Anh, N., Nga, H., & Nguyet, H. (2025). Vegetative Propagation of Phalaenopsis Sogo Yukidian. Vietnam Journal of Agricultural Sciences, 12(8), 1283–1293. https://doi.org/10.31817/tckhnnvn.2014.12.8.

Vegetative Propagation of Phalaenopsis Sogo Yukidian

Nguyen Thi Son (*) , Nguyen Quang Thach , Nguyen Thi Ly Anh , Hoang Thi Nga , Hoang Thi Anh Nguyet

  • Tác giả liên hệ: [email protected]
  • Keywords

    Nodal bud, Phalaenopsis Sogo Yukidian, propagations, protocorms

    Abstract


    Phalaenopsis Sogo Yukidian is a beautiful, lovely and high value orchid in the world and also in Vietnam. An in vitro culture protocol for rapid propagation was established to multiply this valuable flower. Nodal buds of inflorescence of Phalaenopsis Sogo Yukidian were used as initial materials. Optimal sterilization treatment was obtained with CaOCl2 15% for 7 min and then with Presept (Johnson and Johnson) 1% for 3 min. The optimal medium for protocorm regeneration was MS + 2.0 mg/l BA + 6g/l agar. The optimal medium for shoot induction was MS + 0.5mg/l BA + 0.5mg/l α NAA + 6g/l agar. Shoots grew fastest on MS medium supplemented with 10% coconut water. MS medium supplemented with 10% coconut water + 3.0mg/l BA + 6g/l agar was the best medium for shoot multiplication with the multiplication rate of 4.20 times after 6 weeks. MS medium supplemented with 10% coconut water + 0.5 g/l activate charcoal + 6g/l agar was the suitable for rooting.

    References

    Arditti and Ernst (1993). Micropropagation of orchid. New York. John Wiley & Sons, Inc.

    George, EF. and Sherrington, PD. (1984). Plant propagation by Tissue Culture. Eastern Press. England.

    Griesbach, R.J (2002). Development of Phalaenopsis Orchids for the Mass-Market. p. 458-465.

    Ichihashi and Hiraiwa (1996). Effects of solidifier, coconut water and carbohydrate sourse on groeth of embryogennic callus in Phalaenopsis and allied general. J. Orchid Soc. India, 10: 81-88.

    Park et al., (2001). Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell, Tissue and organ Culture, 63: 67-72.

    Parisa Shekarriz, Mohsen Kafi, Shirin Dianati Deilamy, Masoud Mirmasoumi (2014). Coconut water and peptone improve seed germination and protocorm like. Agriculture Science Developments, 3(10): 317-322.

    Polonca KOŠIR (2004). Direct shoot regeneration from nodes of Phalaenopsis orchids. Acta agriculturae slovenica, 83 - 2, november 2004, p. 233 - 242

    Sagawa and Kunasaki (1982). Clonal propagation of orchids by tissue culture. In: Proc. 5th Cong. Plant Tissue and Cell Culture, p. 683-684.

    Tanaka et al., (1974). Studies on the clonal propagation of monopodial orchid by tissue culture. I. Formation of protocorm -like bodies from leaf tissue in Phlaenopsis and Vanda in vitro. J. Jpn. Soc. Hortic. Sci. (Citied in Tanaka et al., 1976).

    Tanaka (1990). Micropropagation of Phalaenopsis through leaf segment culture, In: Proceedings of NIOS 90. Nagoya, p.113-119.

    Zhang et al., (2004). Tissue culture and rapid micropropagation of Phalaenopsis amabilis. Journal of Plant Resources and Environment, 13: 38-40