Development of China Pink (Dianthus chinensis) Polyploid Lines via In Vitro Treatment with Colchicine

Date Received: 16-10-2014

Date Accepted: 24-11-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.8.

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Issue: Vol. 12 No. 8 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Anh, N., Phuong, N., Thanh, H., Ha, L., & Han, N. (2025). Development of China Pink (Dianthus chinensis) Polyploid Lines via In Vitro Treatment with Colchicine. Vietnam Journal of Agricultural Sciences, 12(8), 1322–1330. https://doi.org/10.31817/tckhnnvn.2014.12.8.

Development of China Pink (Dianthus chinensis) Polyploid Lines via In Vitro Treatment with Colchicine

Nguyen Thi Ly Anh (*) , Nguyen Thi Thanh Phuong , Ho Thi Thu Thanh , Le Hai Ha , Nguyen Thi Han

  • Tác giả liên hệ: [email protected]

    • Keywords

    Dianthus chinensis, Tim Vien Trang cultivar, colchicine treatment, polyploidy, mutations

    Abstract


    Colchicine has been successfully used for in vitro treatment for inducton of polyploids in many plants with high efficiency. The aim of this study was to generate new polyploid lines of China pink for breeding and selection in Vietnam. The experiments were carried out on the cultivar Tim Vien Trang of China pink (Dianthus chinensis). In vitro nodal stems were used for treatment with colchicine solution atconcentration of 0, 0.01%, 0.05% and 0.1%  for 24 hours, 48 hours and 72 hours. The mutant types were isolated based on morphological characteristics in in vitro and in vivo conditions. The results showed that the ploidy mutation rate was in a range of 0.87% to 12.85% when the explants were treated in 0.05% and 0.1% of colchicine solution for 24 hours and 48 hours and in 0.01% and 0.05% of colchicine solution for 72 hours. The mutants had highest quality from the treatments of 0.05% and 0.1% of colchicine concentration for 48 hours. Among eight different mutant types, the mutant types D7 and D9 carried several superior morphosological features such as the plant height, leaf size, flower diameter, flower longevity and color intensity in comparison with original cultivar. These two polyploid lines were cloned in vitro for further evaluation of genetic stability.

    References

    Trần Thị Hạnh, Hà Thị Thuý, Đỗ Năng Vịnh (2003). Tạo dòng tứ bội thể ở cam Xã Đoài bằng xử lý colchicine chồi nuôi cấy trong điều kiện in vitro. Báo cáo khoa học Hội nghị Công nghệ sinh học toàn quốc, Nhà xuất bản Khoa học và Kỹ thuật

    Lâm Ngọc Phương và Nguyễn Kim Hằng (2010). Tạo cây dưa hấu tứ bội bằng xử lý colchicine in vitro. Tạp chí Khoa học, 16a: 234-244.

    Trần Duy Quý (1997). Đột biến cơ sở khoa học và ứng dụng. Nhà xuất bản Nông nghiệp, Hà Nội, tr. 46-61.

    Lê Duy Thành (2001). Cơ sở di truyền chọn giống thực vật, Nhà xuất bản Khoa học và Kỹ thuật, tr. 67 - 97

    Nguyễn Thị Ngọc Trâm, Trần Nhân Dũng và Đỗ Tấn Khang (2012) . Đánh giá hiệu quả của colchicine trong chọn tạo giống quýt hồng Lai vung tứ bội (Citrus reticulata Blanco). Tạp chí Khoa học, 23a: 174-183.

    Ali, M., Okubo, H. and Fujieda, K. (1992). Production and characterization of Solanum amphidiploids and their resistance to bacterial wilt. Scientia Hortic., 49: 181-196.

    Beyene Demtsu, Thunya Taychasinpitak, Shermarl Wongchaochant, Benya Manochai (2013). Induced Mutation by Colchicine Treatment of Somatic Embryos in ‘Namwa’ Banana (Musa sp. ABB). International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 4(4): 311-320.

    Chakraborti SP, Vijayan K, Roy BN, Qadri SMH (1998). In vitro induction of tetraploidy in mulbery (Morus alba L.). Plant Cel Rep., 17: 79-803.

    Dolezˇel J, Binarova´ P, Lucretti S. (1989). Analysis of nuclear DNA content in plant cells by flow cytometry. Biologia Plantarum, 31: 113-120.

    Gamborg OL, Phillips GC (1995). Plant cell, tissue and organ culture: Fundamental methods. Springer-Verlag Berlin Heidelberg GmbH, p. 358.

    Ganga, M., and N. Chezhiyan (2002). Influence of the antimitotic agents colchicine and oryzalin on in vitro regeneration and chromosome doubling of diploid bananas (Musa spp.), J. Hortic. Sci. Biotechnol., 77(5): 572-575.

    Hasan Abu-Qaoud and Munqez J. Y. Shtaya (2014). The Efect of Colchicine on Adventitious Shoot Regeneration from Cultured Leaf Explants of Petunia hybrida. British Biotechnology Journal, 4(5): 531-540.

    Höfer M, Meister A (2010). Genome size variation in Malus species. J. Bot., 2010: 1-8.

    Ishizaka, H. and Uematsu, J. (1994). Amphidiploids between Cyclamenpersicum Mill. and C. hederifolium Aiton induced through colchicine treatment of ovules in vitro and plants. Breed. Sci., 44: 161-166.

    Kaewpoo M, Te-chato S. (2010). Study on ploidy level of micropropagated Jatropha curcas L. via flow cytometry. J. Agric. Technol., 6(2): 391-400.

    Kim MS and Kim JY (2003). Chomosome doubling of a Cymbidium hybrid whit colchicine treatment in meristem culture. Proc. Nioc., 1: 37-40.

    Kubalakova M, Vrana J, Cihalikova J, Simkova H, Dolezel J (2002). Flow karyotyping and chromosome sorting in bread wheat (Triticum aestivum). Theor. Appl. Genet., 104(8): 1362-1372.

    Madon M, Clyde MM, Hasmim H, Mohd YY, Mat H, Saratha S (2005). Poliploidy induction of oil palm though colchicine and oryzalin treatments. J. Oil Palm Res., 17: 110-123.

    Motonobu E, Kim JS, Inada I (1997). Production and characteristics of chromosome doubling plants of small flowered garden Chysanthemum, Dendranthema x Gradiflorum Ramat. Kitam. cv. YS by colchicine treatment of cultured shoot tips. J. Jpn Soc. Hortic. Sci., 65: 528-833.

    Murashige T. and Skoog F., (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.

    Pinheiro AA, Pozzobon MT, Valle CB, Penteado MIO, Caneiro VTC (2000). Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep., 19(3): 274-278.

    Sajad Y,Jaskani MJ, Mehmod A, Ahmad I, Abas H (2013). Efect of colchicines on in vitro polyploidy induction in African marigold (Tagetes erecta). Pak. J. Bot., 45(3): 125- 58.

    Sakhanokho HF., Rowena KRY, Islam-Faridi KN (2009). Induced polyploidy in diploid ornamental ginger (Hedychium muluense R. M. Smith) using colchicine and oryzalin. Hortscience, 44(7): 1809-1814.

    Carine Simioni and Cacilda Borges do Valle (2009). Chromosome duplication in Brachiaria (A. Rich.) Stapf allows intraspecific crosses. Crop Breeding and Applied Biotechnology, 9: 328-334.

    Souza FF, Queiróz MA (2004). Avaliação de caracteres morfológicos úteis na identificação de plantas poliploides de melancia. Hort. Bras., 22(3): 516-520.

    Thao, N.T.P., K. Ureshino, I. Miyajima, Y. Ozaki, and H. Okubo (2003). Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss. Org. Cult., 72: 19-25.

    Vichiato MRM (2004). Indução de tetraploides e alongamento de plantas de Dendrobium nobili LINDL. (Orchidaceae). Thesis, Universidade Federal de Lavras, Lavras.

    Xing SH, Xin-Bo G, Quan W, Qi-Fang P, Yue-Sheng T, Pin L, Jing-Ya Z, Guo-Feng W, Xiao-Fen S, Ke-Xuan T (2011). Induction and flow cytometry identification of tetraploids fromseed-derived explants through colchicine treatments in Catharanthus roseus (L.) G. Don. J. Biomed. Biotechnol., 1: 1-10.

    Watrous, S.B. and Wiiber, D.E. (1988). Artificial induction of polyploidy in Paphiopedilum. Lmdleyana, 3: 177-183.