Isolation, Screening and Identification of Erwinia Carotovora subsp. carotovora Causing Soft Rot Disease on Some Crops

Date Received: 20-10-2016

Date Accepted: 09-11-2017

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2017.15.10.

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Issue: Vol. 15 No. 10 (2017)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Canh, N., Huong, T., & Dao, T. (2025). Isolation, Screening and Identification of Erwinia Carotovora subsp. carotovora Causing Soft Rot Disease on Some Crops. Vietnam Journal of Agricultural Sciences, 15(10), 1401–1409. https://doi.org/10.31817/tckhnnvn.2017.15.10.

Isolation, Screening and Identification of Erwinia Carotovora subsp. carotovora Causing Soft Rot Disease on Some Crops

Nguyen Xuan Canh (*) , Trinh Thi Huong , Tran Thi Dao

  • Tác giả liên hệ: [email protected]

    • Keywords

    Soft rot disease, bacteria, Erwinia carotovora, ATCC15713

    Abstract


    Soft rot disease caused by Erwinia carotovora is commonly encountered in many crops. In this study, 41 different diseased plant samples from Ha Noi, Bac Ninh, Hung Yen, Bac Giang, and Ninh Binh provinces were collected. By preliminary screening 29 bacterial strains were isolated. Based on physiological and biochemical characteristics, four strains, M12-2, M17-4, M21-4 and M12ydc showing similarities with Erwinia carotovora subsp. carotovora ATCC15713 strain were identified. These strains were capable of causing soft rot in potato, cabbage and tomato by artificial inoculation in the laboratory. PCR analysis using specific primers of Ec001 indicated that these strains carried virulent gene like Erwinia carotovora subsp. carotovora ATCC15713.

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