Date Received: 28-07-2014
Date Accepted: 18-09-2014
Date Published: 06-08-2025
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.
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How to Cite:
Study on Drought Tolerance of Some Local Upland Rice Varieties (Oryza sativa L.)
,
Nguyen Nhu Khanh
,
Chu Hoang Mau
Keywords
Drought tolerance, LTPs, upland rice
Abstract
Increased drought in many parts of the world is a major cause of promoting the projects and researches to develop drought-resistant crops with high productivity. A great deal of researches focused on identifying morphological characteristics, biochemical and physiological parameters. Besides, studies on the nature of drought tolerance at the molecular level have been scientists’ interest. Drought tolerance of 25 local upland rice varieties at the seedling stage was identified, wherein Giang Bau was found to show the best drought tolerance variety while Khau lay khao exhibited lowest degree of drought tolerance. The use of RAPD marker with 20 random primers revealed genetic relationship of 25 local upland rice varieties with the coefficients of similarity among upland rice cultivars ranging from 79% to 92%. LTP coding genes of two local upland rice varieties with different drought resistance was isolated and identified. The comparison and analysis of LTP gene sequences among two local upland rice varieties (Giang bau and Khau lay khao) and a Japanese rice variety (Yukihikari) showed that the genetic similarity of LTP gene sequences between Yukihikari and Giang bau is 100% and the genetic similarity of LTP gene sequences between Giang bau and Khau lay khao is 98.1%. Comparing amino acid sequences of LTP proteins among these three varieties showed an increase of amino acid valine residue in Khau lay khao’s LTP protein and an increase of the amino acid leucine residue in Giang bau’s LTP protein.
References
Arunyanark, A; Jogloy, S; Akkasaeng, C; Vorasoot, N; Kesmala, T; Nageswara Rao, R. C; Wright, G. C;Patanothai, A. (2008). Chlorophyll stability is an indicator of drought tolerance in peanut. Journal of Agronomy and Crop Science, 194(2): 113-125.
Lê Trần Bình, Lê Thị Muội (1998). Phân lập gen và chọn dòng chống chịu ngoại cảnh bất lợi ở cây lúa. Nhà xuất bản Đại học Quốc gia, Hà Nội.
Trần Văn Đạt (2005). Sản xuất lúa gạo thế giới: Hiện trạng và khuynh hướng phát triển trong thế kỷ 21. Nhà xuất bản Nông nghiệp.
Gawel N.J. and Jarret R.L. (1991). A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Molecular Biology Reporter, 9: 262-266.
M. Hassanzadeh, A. Ebadi, M. Panahyan-e-Kivi, A.G. Eshghi, Sh. Jamaati-e-Somarin, M. Saeidi and R. Zabihi-e-Mahmoodabad. (2009). Evaluation of Drought Stress on Relative Water Content and Chlorophyll Content of Sesame (Sesamum indicum L.) Genotypes at Early Flowering Stage. Research Journal of Environmental Sciences. (3): 345-350.
Jean-Claude Kader (1996). Lipid-transfer proteins in plants. Annual Reviews. Plant Physiology, 47: 627-654.
Trần Thị Phương Liên, Lê Thị Thu Hiền, Nguyễn Đăng Tôn, Cao Xuân Hiếu, Nông Văn Hải, Lê Thị Muội, Trần Đình Long (2003). Nghiên cứu sự đa dạng của gen chaperonin CCT ở cây đậu tương. Tạp chí Sinh học, 25(3): 77-82.
Longenberger, Polly; Smith, Wayne; Burke, John; McMichael, Bobbie (2007). Drought tolerance classification via chlorophyll fluroescence in upland cotton (Gossypium hirsutum L.). Characterization and enhancement of plant resistance to water-deficit and thermal stresses. Plant Stress and Germplasm Development. ASA-CSSA-SSSA Annual Meeting Abstracts.
Masuta, C., Furuno, M., Tanaka, H., Yamada, M. and Koiwai, A. (1992). Molecular cloning of a cDNA clone for tobacco lipid transfer protein and expression of the functional protein in Escherichia coli. FEBS Lett, 311 (2): 119-123
Chu Hoàng Mậu, Nguyễn Thị Thu Hiền (2007). Đánh giá khả năng chịu hạn và tách dòng gen mã hoá protein dehydrin (LEA-11) của một số giống đậu tương (Glycine max L.) địa phương miền núi. Tạp chí Sinh học, 29 (4): 31-41.
Chu Hoàng Mậu, Nguyễn Vũ Thanh Thanh, Trần Thúy Liên, Nguyễn Thị Mai Lan (2009). Tách dòng gen LTP (Lipid transfer proteins) của cây đậu xanh, Tạp chí Khoa học và Công nghệ-Đại học Thái Nguyên, 52 (4): 94-98.
Mukai, T., Sakaki, T. and Akiyama,T. (2003). A gene coding for putative lipid transfer protein (LTP) is down-regulated by drought stress, ABA and methyl jasmonate in ric. (Oryza sativa L.). Oryza sativa (japonica cultivar-group) lipid transfer protein-like protein (LTP1) mRNA, complete cds. AY466108. Http: //www.ncbi.nlm.nih.gov.
Rohlf FJ, (2000). NTSYS-pc: Numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, New York.
Sambrook J., Russell D.W. (2001). Molecular Cloning. A Laboratory Manual. 3rd Edition, Cold Spring Haror Laboratory Press, Cold Spring Harbor, NY.
Somerville C, Briscoe J (2001). Genetic engineering and water. Science, 292: 2217.
Tchang, F., This, P., Stiefel, V., Arondel, V., Morch, M.-D., Pages, M., Puigdomenech, P. Grellet, F., Delseny, M., Bouillon, P., Huet, J.-C., Guerbette, F., Beauvais-Cante, F., Duranton, H., Pernollet, J.-C. and Kader, J.-C. (1988). Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. Amino acid sequence homologies between plant phospholipid transfer proteins. J. Biol. Chem., 263 (32): 16849-16855
Trần Nguyên Tháp (2001). Nghiên cứu xác định một số đặc trưng của các giống lúa chịu hạn và chọn tạo giống lúa chịu hạn CH5. Luận án Tiến sỹ Nông nghiệp. Viện Khoa học Kỹ thuật Nông nghiệp Việt Nam.
Torres-Schumann, S., Godoy, J.A. and Pintor-Toro, J.A. (1992). A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol. Biol, 18 (4): 749-757.
Thoma, S., Hecht, U., Kippers, A., Botella, J., De Vries, S. and Somerville, C. (1994). Tissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis. Plant Physiol, 105 (1): 35-45
Zhang JZ, Creelman RA, Zhu J-K. (2004). “From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops”. Plant Physiol, 135: 615-621.