Date Received: 12-01-2026
Date Accepted: 09-04-2026
Date Published: 25-06-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.6.02
Views
Downloads
How to Cite:
Study on the Fungus Agroathelia rolfsii YBS24 Causing Basal Stem, Root Rot Disease of Cassava
Keywords
Agroathelia rolfsii, basal stem and root rot disease, cassava, Manihot esculenta Crantz
Abstract
Basal stem and root rot is one of the most destructive diseases causing serious losses in both yield and quality of cassava in many growing regions of Vietnam, particularly in the northern mountainous provinces. This study was conducted to identify the causal agent of cassava basal stem and root rot in Lao Cai province, Vietnam, and to evaluate its biological characteristics. Isolation, artificial inoculation, morphological characterization, and internal transcribed spacer (ITS) region sequence analysis were used in this study. The results indicated that isolate Agroathelia rolfsii YBS24 is the causal pathogen of basal stem and root rot of cassava. The fungus exhibited optimal growth on PDA medium. The fungus grew over a wide pH range, from 4 to 9, with optimal growth between pH 5 and 8. It also grew over a relatively wide temperature range of 15-35°C, with temperatures between 25°C and 30°C being most favorable for mycelial growth. The results of this study provide a crucial scientific foundation for developing effective and sustainable management strategies for cassava basal stem and root rot disease.
References
Athipunyakom P., Seemadua S., Doungsa-ard C. & Kitjaideaw A. (2019). การ ศึกษา สาเหตุ อาการ โคน เน่า และ หัว เน่า ของ มัน สำปะหลัง โดย ศึกษา ลักษณะ ทาง สัณฐาน วิทยา และ ชีว โมเลกุล. Thai Agricultural Research Journal. 37(3): 307-319. Bandyopadhyay R., Mwangi M., Aigbe S.O. & Leslie J.F. (2006). Fusarium species from the cassava root rot complex in West Africa. Phytopathology. 96: 673-676. Banito A., Kpémoua K. E., Bissang B. & Wydra K. (2010). Assessment of cassava root and stem rots in ecozones of Togo and evaluation of the pathogen virulence. Pakistan Journal of Botany. 42(3): 2059-2068. Boari A.D.J., Cunha E.M., Quadros A.F.F., Barreto R. W. & Fernandes A.F. (2018). First report of Phytopythium sp. causing storage root rot and foliage blight of cassava in Brazil. Plant Disease. 102(5): 1042-1042. Boas S.A.V., Hohenfeld C.S., de Oliveira S.A.S., da Silva Santos V. & de Oliveira E.J. (2016). Sources of resistance to cassava root rot caused by Fusarium spp.: a genotypic approach. Euphytica. 209(1): 237-251. Burgess L.W., Knight T.E., Tesoriero L. & Phan H.T. (2008). Diagnostic manual for plant diseases in Vietnam. ACIAR, Canberra. pp. 126-133. Doyle J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical bulletin. 19: 11-15. Guo H., Li C.P., Shi T., Fan C.J. & Huang G.X. (2012). First report of Phytophthora palmivora causing root rot of cassava in China. Plant Disease. 96(7): 1072-1072. Kumar S., Stecher G., Li M., Knyaz C. & Tamura K. (2018). MEGA X: Moleculacar Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution. 35: 1547-1549. Machado A.R., Pinho D.B., de Oliveira S.A., & Pereira O.L. (2014). New occurrences of Botryosphaeriaceae causing black root rot of cassava in Brazil. Tropical Plant Pathology. 39: 464-470. Muniz M.D.F.S., Andrade F.W.R.D., Queiroz F.M., Moura Filho G. & Menezes M. (2006). Caracterização de isolados de Phytophthora drechsleri, agente causal da podridão mole de raízes de mandioca. Fitopatologia Brasileira. 31: 195-198. Okechukwu R.U., Dixon A.G., Akoroda M.O., Mwangi M. & Bandyopadhyay R. (2009). Root rot resistance in new cassava varieties introduced to farmers in Nigeria. Experimental Agriculture. 45(1): 15-24. Onyeka T.J., Ekpo E.J.A. & Dixon G.O. (2001). Prevalence of cassava root rot disease and the associated pathogens in Nigeria. In Book of Abstracts, Eighth Triennial Symposium of the International Society for Tropical Root Crops-African Branch. International Institute of Tropical Agriculture, Ibadan, Nigeria. pp. 12-16. Onyeka T.J., Dixon A.G.O. & Ekpo E.J.A. (2005). Identification of levels of resistance to cassava root rot disease (Botryodiplodia theobromae) in African landraces and improved germplasm using in vitro inoculation method. Euphytica. 145(3): 281-288. Pham C.V. & Tran H.T. (2021). Cunninghamella elegans causing cassava root rot in Vietnam. Australasian Plant Disease Notes. 16(1): 14. Ubalua A.O. & Oti E. (2008). Evaluation of antimicrobial properties of some medicinal plants for fresh cassava roots preservation. Pakistan Journal of Nutrition. 7(5): 679-681.
Viện Bảo vệ thực vật (2023). Phương pháp điều tra, chẩn đoán giám định một số loài sinh vật gây hại quan trọng trên cây trồng và nấm ăn ở Việt Nam. Nhà xuất bản Nông nghiệp. 290 trang.
Võ Thị Thu Oanh & Lưu Từ Đoan Trang (2017). Đánh giá khả năng đối kháng của một số dòng Trichoderma đối với Phytopythium helicoides trong điều kiện phòng thí nghiệm. Tạp chí Nông nghiệp và Phát triển. 16(5): 1-8. White T.J., Bruns T.D., Lee S. & Taylor J.W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics in PCR Protocols: a Guide to Methods and Applications, eds Innis M.A., Gelfand D.H., Sninsky J., White T.J. California, CA: San Diego, Academic. pp. 315-322. Zinsou V.A., Afouda L.A.C., Ahohuendo B.C. & Wydra K. (2017). Importance of fungal root rot pathogens of cassava in Benin. Pakistan Journal of Botany. 49(5): 2023-2028.