Date Received: 30-01-2026
Date Accepted: 07-05-2026
Date Published: 28-07-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.7.05
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Effect of Carrier Materials and Storage Temperature on the Viability of freeze-dried Lactobacillus plantarum LAB8.1CM in Powder Form
,
Tran Trung Giang
1
,
Phan Thi Cam Tu
1
,
Huynh Truong Giang
1
Keywords
Carrier material, density, Lactobacillus, protease activity, temperature
Abstract
The study aimed to evaluate the effects of storage temperature (4°C and 30°C) and carrier materials (dextrose, lactose, skim milk, and rice bran) on the viability and enzyme activity of strain Lactobacillus plantarum LAB8.1CM in powder formulations after 90 days of storage. The results showed that the viability of probiotic was consistently better maintained at 4°C across all carrier materials compared with storage at 30°C. Among these carriers, skim milk exhibited the highest protective capacity at 4°C, with a minimal cell death rate of 0.004 ± 0.002/day, while storage at 30°C resulted in a considerable increase to an average of 0.05 ± 0.002/day. In contrast, dextrose showed the poorest protective performance, with cell death rates of 0.017 ± 0.002/day at 4°C and 0.062 ± 0.001/day at 30°C. In addition, protease activity gradually decreased during storage; however, enzyme activity was maintained at approximately twofold higher levels at 4°C compared with 30°C. Notably, no statistically significant differences (P >0.05) in protease activity were observed among the various carrier materials, and no significant interaction was identified between storage temperature and carrier type (P >0.05). Overall, storage at low temperatures in combination with suitable carrier materials plays a crucial role in preserving the viability and biological activity of Lactobacillus sp. in powdered probiotic formulations under experimental conditions.
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