Evaluation of the Ability to Detect Antibodies Against African Swine Fever Virus  by the Elisa Method

Date Received: 14-05-2025

Date Accepted: 09-03-2026

Date Published: 28-03-2026

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.3.04

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Issue: Vol. 24 No. 3 (2026): Tạp chí Khoa học Nông nghiệp Việt Nam

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hanh, V., Hiep, V., Hung, L., Phuong, N., Hoa, N., Huy, D., … Huong, L. (2026). Evaluation of the Ability to Detect Antibodies Against African Swine Fever Virus  by the Elisa Method. Vietnam Journal of Agricultural Sciences, 24(3), 319–328. https://doi.org/10.31817/tckhnnvn.2026.24.3.04

Evaluation of the Ability to Detect Antibodies Against African Swine Fever Virus  by the Elisa Method

Vu Duc Hanh , Vu Lai Xuan Hiep 1 , Luong Quoc Hung 1 , Nguyen Thi Minh Phuong 2 , Nguyen Thi Hoa 2 , Duong Nhat Huy 3 , Bui Minh Huy 2 , Dang Thi Thuy Linh 2 , Nguyen Thi Phuong Dong 2 , Pham Hong Trang 2 , Nguyen Van Giap 2 , Lai Thi Lan Huong (*)

  • Tác giả liên hệ: [email protected]
  • 1 Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 3 Công ty cổ phần Hưng Gia Nam

    • Keywords

    African swine fever, in dirct ELISA, serum, oral fluid

    Abstract


    The present study was conducted to evaluate the ability of indirect ELISA (iELISA) method to detect antibodies against African Swine Fever Virus (ASFV) using recombinant p30 protein as the coating antigen in serum and oral fluid samples. A total of 84 paired samples (each pair consisting of serum and oral fluid collected from the same individual) were obtained, including 26 negative samples and 58 samples collected from ASF-affected herds (25 acute and 33 chronic cases). The evaluation results showed that the iELISA method achieved a diagnostic specificity of 100% for both serum and oral fluid samples without cross-reactivity or false-positive results observed. When compared with the commercial reference ELISA kit (Ingezim PPA Compac), the iELISA demonstrated high agreement in serum samples, with a concordance rate of 90.47% and a Cohen’s Kappa coefficient of 0.81. When comparing paired samples from the same individual, antibody levels in oral fluid showed a strong positive linear correlation with those in serum (r = 0.765, P <0.001), along with substantial diagnostic agreement (Kappa = 0.73). Moreover, the iELISA method in both sample matrices reflected the trend of immune response dynamics across disease stages, as indicated by an increase in S/P values from the acute to the chronic phase.

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