PROPAGATION OF Plantago major L. BY PLANT TISSUE CULTURE TECHNIQUE

Ngày nhận bài: 29-09-2016

Ngày duyệt đăng: 20-12-2016

Ngày xuất bản: 06-08-2025

DOI: https://doi.org/10.31817/tckhnnvn.2017.15.3.

Số: Tập 15 Số 3 (2017)

Chuyên mục:

KỸ THUẬT VÀ CÔNG NGHỆ

Cách trích dẫn:

Ay, N., V.Duy, M., Baatartsogt, O., Altantsetseg, K., & Enkhchimeg, V. (2025). PROPAGATION OF Plantago major L. BY PLANT TISSUE CULTURE TECHNIQUE. Tạp Chí Khoa học Nông nghiệp Việt Nam, 15(3), 282–289. https://doi.org/10.31817/tckhnnvn.2017.15.3.

PROPAGATION OF Plantago major L. BY PLANT TISSUE CULTURE TECHNIQUE

N. V. Ay 1, 2 , M. V.Duy 1, 2 , O. Baatartsogt 1, 2 , Kh. Altantsetseg 1, 2 , V. Enkhchimeg (*) 1, 2

  • Tác giả liên hệ: [email protected]
  • 1 Dept of Biotechnology and breeding, School of Animal Sciences and Biotechnology, Mongolian University of Life Sciences, Mongolia
  • 2 College of Agriculture and Applied Biology, Can Tho University, Vietnam

    • Từ khóa

    2, 4-D: 2, 4-Dichlorophenoxyacetic acid, ABBREVIATIONS, Acclimatization, BA: Benzyl adenine, NAA: α-Naphthaleneacetic acid, MS: Murashige and Skoog, Plantago major L, plant growth hormone, TDZ: Thidiazuron, tissue culture

    Tóm tắt


    Plantago major L., a species that originated in the Far East, has been used for medicinal aspects since ancient times. The technique for callus induction, growth, and plant regeneration from cultured young leaves and petiole explants of Plantago major was carried out. The best initiation and growth of calli was achieved on MS supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA. Shoot regeneration from the calli was obtained on MS medium containing
    1 mgL-1 TDZ alone or combined with 0.5 mgL-1 α-NAA (100 and 96%, respectively). Moreover, the optimum medium for shoot multiplication was MS medium comprising 5 mgL-1 BA, and root formation from the shoots was half-strength MS medium supplemented with 2 mgL-1 NAA. The regenerated plants were successfully transferred into pots containing a mixture of decayed straw: rice husk ashes, (1:1, v/v), sand: soil (1:1, v/v), soil, or sand and grown in nethouse. The phenotypes of regenerated plants were normal as a mother plant.

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