The Initial Protocol of Micropropagation in Angel Flower (Angelonia goyazenzsis Benth)

Date Received: 25-07-2014

Date Accepted: 07-10-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Loc, P., Luan, N., Bien, L., & Lieu, N. (2025). The Initial Protocol of Micropropagation in Angel Flower (Angelonia goyazenzsis Benth). Vietnam Journal of Agricultural Sciences, 12(7), 1120–1124. https://doi.org/10.31817/tckhnnvn.2014.12.7.

The Initial Protocol of Micropropagation in Angel Flower (Angelonia goyazenzsis Benth)

Pham Van Loc (*) , Nguyen Thanh Luan , Lai Dinh Bien , Nguyen Thi Lieu

  • Tác giả liên hệ: [email protected]

    • Keywords

    Angelonia goyazenzsis, adventitious shoot, BA, IBA, micropropagation

    Abstract


    This study aimed to examine initial protocol of micropropagation for angel flower (Angelonia goyazenzsis Benth). Young leaves were cultured on MS medium supplemented with 0.2 mg/l NAA and  BA at different concentrations for shoot induction. The most effective rate of adventitious shoot proliferation was induced on MS medium supplemented with 1.5 mg/l BA while MS medium supplemented with 0.1 mg/l IBA was suitable for in vitro  rooting stage. In vitro derived plantlets were successfully established in the substrate with combination of sand: smoked rice husk in the ratio of 1:1. These results provide the basis for further studies on large-scale rapid propagation of angle flower. 

    References

    Chang H., Charkabarty D., Hahn E. and Paek K. (2003). Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. In Vitro Cellular & Developmental Biology Plant, 39(2): 129-134

    Choi J., Kim H., Lee C., Bae J., Chung Y., Shin J. and Hyung N. (2001). Efficient and simple plant regeneration via organogenesis from leaf segment cultures of persimmon (Diospyros kaki Thunb.). In Vitro Cellular & Developmental Biology Plant, 37(2): 274-279.

    Dias M.C., Almeida R. and Romano A. (2002). Rapid clonal multiplication of Lavandula viridis L’H´er through in vitro axillary shoot proliferation. Plant Cell, Tissue and Organ Culture, 68: 99-102.

    Giovanni I. and Marcello A. (2008). Micropropagation of Metrosideros excelsa. In Vitro Cellular & Developmental Biology Plant, 44: 330-337

    Molla M.M.H., Dilafroza M. K., Khatun M.M., Al-amin M. and Malek M.A., (2004). In vitro rooting and ex vitro plantlet establishment of BARI banana 1 (Musa sp.) as influenced by different concentration of IBA (Indole 3- butyric Acid). Asian Journal of Plant Sciences, 3(2): 196-199.

    Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol., 15: 473-497

    Puhan P. and Rath S. P. (2012). In vitro micropropagation of Desmodium gangeticum (L.) DC (Fam-Fabaceae): a medicinal legume through axillary bud multiplication. Pakistan Journal of Biological Sciences, 15(10): 477-483.

    Thirugnanasampandan R., Mahendran G. and Narmatha B. V., (2010). High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara. Acta Physiol. Plant, 32: 405-409.

    Bùi Trang Việt (2000). Sinh lý thực vật đại cương (Phần II. Phát triển). Nhà xuất bản Đại học Quốc gia Thành phố Hồ Chí Minh, Thành phố Hồ Chí Minh, tr. 88.

    Vinterhalter B., Milošević D., Janković T., Milojević J. and Vinterhalter D. (2012). In vitro propagation of Gentiana dinarica Beck. Central European Journal of Biology, 7(4): 690-697.

    Zubeda C. , Imran F., Waseem A., Hamid R., Bushra M. and Azra Q. (2001). Varietal response of Lycopersicon esculentum L. to callogenesis and regeneration. Journal of biological sciences, 1(12): 1138-1140.