Application of DNA Marker to Evaluate Tomato Genetic Resoure for Ripening Inhibitor Gene and Yellow Leaf Curl Virus Resistance

Date Received: 07-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

Views

5

Downloads

5

Issue: Vol. 11 No. 6 (2013)

Section:

NÔNG HỌC

How to Cite:

Ton, P., Tuyen, K., Hai, T., & Bach, N. (2025). Application of DNA Marker to Evaluate Tomato Genetic Resoure for Ripening Inhibitor Gene and Yellow Leaf Curl Virus Resistance. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Application of DNA Marker to Evaluate Tomato Genetic Resoure for Ripening Inhibitor Gene and Yellow Leaf Curl Virus Resistance

Phan Huu Ton (*) , Khuc Ngoc Tuyen , Tong Van Hai , Nguyen Duc Bach

  • Tác giả liên hệ: [email protected]

    • Keywords

    Begomoviruses, DNA marker, ripening inhibitor gene, tomato

    Abstract


    In order to develop the new tomato varieties with delayed ripening and strong resistance to yellow leaf curl disease, evaluation and identification of the genetic resources bearing desirable genes play a very important role. In this study, 200 tomato accessions were evaluated for bio-agronomic characteristics, yield potential, delayed ripening ability and resistance to yellow leaf curl disease by artificial infection. Application of DNA markers was carried out to identify the resistant genes Ty1, Ty2, Ty3 and ripening inhibitor gene (rin) in 200 tomato accessions. Results indicated that seven tomato accessions carry resistance gene Ty1, 7 accessions contain Ty3 gene, of which 2 accessions carrying both Ty1 and Ty3 gene, and 6 accessions carry rin gene. Almost the accessions containing Ty1 and Ty3 were strongly resistant to the virus isolates collected from Hanoi, Bacgiang and Hungyen provines. We also found 15 accessions with slow ripening ability and long shelf life, in which 6 accessions containing ripening inhibitor gene (rin).

    References

    Anbinder I., Reuveni M., Azari R., Paran I., Nahon S., Shlomo H., Chen L., Lapidot M., and Levin I. (2009). Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum. Theoretical and Applied Genetics, 119(3): 519–530.

    Castro A. P. D., Blanca J. M., Díez M. J., and Vinals F. N. (2007). Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato. European Journal of Plant Pathology, 117: 347-356.

    Doyle J. J., and Doyle J. L. (1990). A rapid total DNA preparation procedure for fresh plant tissue. Focus:, 12: 13-15.

    Foolad M. R. (2007). Genome Mapping and Molecular Breeding of Tomato. International Journal of Plant Genomics, 2007.

    Garcia B. E., Graham E., Jensen K. S., Hanson P., Mejía L. and Maxwell D. P. (2007). Co-dominant SCAR marker for detection of the begomovirus-resistance Ty-2 locus derived from Solanum habrochaites in tomato germplasm. Report of the Tomato Genetics Cooperative, 57: 21-24.

    Garcia B. E., and Maxwell D. P. (2011). Evaluation of the JB1 marker for detection of the Ty1 introgression in chromosome 6.

    Garg N., Cheema D. S. and Dhatt A. S. (2008). Utilization of rin, nor, and alc Alleles to Extend Tomato Fruit Availability. International Journal of Vegetable Science, 14(1): 41-54.

    Hanson P. M., Green S. K. and Kuo G. (2006). Ty-2 gene on chromosome 11 conditioning geminivirus resistance in tomato. Report of the Tomato Genetics Cooperative, 56: 17-18.

    Ji Y., and Scott J. W. (2006). Ty-3, a begomovirus resistance locus linked to Ty-1 on chromosome 6 of tomato. Report of the Tomato Genetics Cooperative, 56: 22-23.

    Ji Y., Betteray B. V., Smeets J., Jensen K. S., Mejía L., Scott J. W., Havey M. J. and Maxwell D. P. (2007). Co-dominant SCAR Marker, P6-25, for Detection of Ty-3, Ty-3a, and Ty3b introgressions from three Solanum chilense accessions at 25 cM of Chromosome 6 of Begomovirus-Resistant Tomatoes.

    Ji Y., Scott J. W. and Schuster D. J. (2008). Ty-4, a tomato yellow leaf curl virus resistance gene on chromosome 3 of tomato. Report of the Tomato Genetics Cooperative, 58: 29-31.

    Lapidot M., and Friedmann M. (2002). Breeding for resistance to whitefly-transmitted geminiviruses. Annals of Applied Biology, 140: 109-127.

    McGlasson W. B., Sumeghy J. B., Morris L. L., McBridge R. L., Best D. J. and Tigchelaar E. C. (1983). Yield and evaluation of F1 tomato hybrids incorporating the non-ripening nor gene. Australian Journal of Experimental Agriculture and Animal Husbandry, 23:106–112.

    Nguyễn Thơ (1984). Điều tra nghiên cứu một số bệnh virus chủ yếu của 3 cây trồng họ cà (solanaceae) có ý nghĩa kinh tế: thuốc lá, cà chua, khoai tây (Luận án PTS).

    Vrebalov J., Ruezinsky D., Padmanabhan V., White R., Medrano D., Drake R., Schuch W. and Giovannoni J. (2002). A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus. Science, 296: 343-346.

    Zamir D., Michelson I., Zakay Y., Navot N., Zeidan N., Sarfatti M., Eshed Y., Harel E., Pleban T., van-Oss H., Kedar N., Rabinowitch H. D. and Czosnek H.. (1994). Mapping and introgression of a tomato yellow leaf curl virus tolerance gene Ty-1. Theor. Appl. Genet, 88: 141–146.

    Zhang X. (2010). The development of longer shelf-life gene marker and assisted selection of tomato inbredlines. (Master Molecular vegetable breeding), Huazhong Agricultural University, Wuhan, China.