Morphological Characteristics and Culture Conditions for Mycelial Growth and Fruiting of Black Lingzhi Mushroom (Amauroderma rugosum) Am20

Date Received: 07-02-2025

Date Published: 21-02-2025

##submissions.doi##: https://doi.org/10.1234/wpn00y41

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Issue: Vol. 22 No. 10 (2024)

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Ha, T., Nhàn, L., & Giang, N. (2025). Morphological Characteristics and Culture Conditions for Mycelial Growth and Fruiting of Black Lingzhi Mushroom (Amauroderma rugosum) Am20. Vietnam Journal of Agricultural Sciences, 22(10). https://doi.org/10.1234/wpn00y41

Morphological Characteristics and Culture Conditions for Mycelial Growth and Fruiting of Black Lingzhi Mushroom (Amauroderma rugosum) Am20

Tran Thu Ha , Le Thanh Nhàn , Nguyen Van Giang (*)

  • Tác giả liên hệ: nvgiang@vnua.edu.vn

    • Keywords

    Amauroderma rugosum, pH, temperature, cereal grains, substrates, mycellial growth, fruiting body formation

    Abstract


    Amauroderma rugosum contains various biologically active compounds and is considered a traditional medicine in many Asian countries. However, there was little research on this mushroom species in the world and in Vietnam. This study aimed to describe the morphology, analyze the phylogeny, and optimize the conditions for culturing the mycelium and fruiting bodies of the strain Am20 collected in Dong Nai, Vietnam. The results of the morphological study, combined with molecular data on the ITS region sequence showed that the strain Am20 had high similarity to other strains of A. rugosum. The culturing results showed the mycelia of A. rugosum strain Am20 grew well in a PDA medium at pH 6-7 and a temperature of 30°C. Mycellia of this strain could be cultured on the cereal grains such as rice and wheat. The A. rugosum strain Am20 was able to form and develop fruiting bodies on substrates containing sawdust and corn cobs. The most suitable substrate was 89% sawdust, 10% rice bran, and 1% powder CaCO3. The yield reached 21.1 ± 2.45 g/kg dry substrates.

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