In vitro propagation of papaya Carica papapaya L.

Date Received: 06-06-2025

Date Published: 13-06-2025

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Nam, D., Thuy, N., Hong, N., Ngoc, P., Hoan, N., & Thao, N. (2025). In vitro propagation of papaya Carica papapaya L. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

In vitro propagation of papaya Carica papapaya L.

Do Van Nam , Nguyen Thi Thuy , Nguyen Thi Bich Hong (*) , Pham Thi Ngoc , Nguyen Van Hoan , Nguyen Thi Phuong Thao

  • Tác giả liên hệ: [email protected]
  • Keywords

    BA, α- NAA, IBA, Carica papaya L, in vitro propagation

    Abstract


    This study was conducted to establish the protocol for in vitro propagation of papaya using stem segments. Results showed that double sterilization of explants with HgCl2 0.1% first  for 10 minutes and follwed by 5 minutes was most appropriate. MS medium containing 1 mg/l α-NAA+ 0,5 mg/l BA was optimal for callus induction (90.74%) of young stem slices after 4 weeks of culture. The highest shoot multiplication rate (3.17) was obtained on MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l α- NAA after 4 weeks. Root induction medium was ½ MS containing 1.0 mg/l activated charcoal and 1.5 mg/l IBA. The average rooting percentage  was 50% within 4 weeks.

    References

    Islam R., Rahman S.M., Hosain M. and Joarder O.I. (1993). In vitro clonal propagation of papaya through culture of lateral bubs from mature field- grown plants, Plant tisue culture, 3(1):

    -50.

    Jordan M., Cortes I. and Montenegro G. (1983). Regeneration of plants by embryogenesis from callus cultures of Carica candamarcensis. Plant Sci., Let, 28: 321-326.

    Modal M., Gupta S. and Mukherjee B.B. (1990). In vitro propagation of shoot bubs of Carica papaya L. (Caricaceae) var. Honey Dew. Plant cell Rep., 8: 609-612.

    Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15(3): 473-497.

    Nguyễn Thị Nhẫn (2004). Nghiên cứu quy trình nhân nhanh in vitro cây đu đủ (Carica papaya L.). Tạp chí KHKT Nông Nghiệp, 2(3): 174-175.

    Protual K.R., Shyamal K. R. and Lokman Hakim M.d. (2012). Propagation of Papaya (Carica papaya L.) CV. Shahi through in vitro culture. Banglades J., Bot, 41 (2): 191- 195.

    Rahman S.M., Hossain M., Joarder O.I. and Islam R. (1992). Rapid clonal propagation of papaya through culture of shoot apices. Indian J. Hort., 49: 473-497.