Construction of Vector Containing Artificial miRNA to Specifically Inhibit MPG1 Gene Expression in Magnaporthe grisea Causing Rice Blast

Date Received: 02-07-2025

Date Published: 02-07-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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NÔNG HỌC

How to Cite:

Thao, N., Hieu, N., Huong, P., & Linh, N. (2025). Construction of Vector Containing Artificial miRNA to Specifically Inhibit MPG1 Gene Expression in Magnaporthe grisea Causing Rice Blast. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Construction of Vector Containing Artificial miRNA to Specifically Inhibit MPG1 Gene Expression in Magnaporthe grisea Causing Rice Blast

Nguyen Thi Phuong Thao (*) , Nguyen Trang Hieu , Phan Thi Huong , Nguyen Thi Thuy Linh

  • Tác giả liên hệ: [email protected]

    • Keywords

    MPG1 gene, miRNA, Magnaporthe grisea, transgenic rice

    Abstract


    In hot and humid climate countries, such as Vietnam, Magnaporthe grisea causes serious damage to rice. MPG1, a pathogenicity gene of Magnaporthe grisea is related to the process of aspersorium formation, symptom development and sporulation. Therefore, inhibition of MPG1 gene expression probably results in reduced or loss of pathogenicity of Magnaporthe grisea. A coding fragment of MPG1 gene of 4 rice blast fungus isolates was cloned and the sequencing results showed that the values of gene identity ranged from 99.3-100% among isolates in comparison with the control strain (Guy-11, ID genbank: L20685.2). Two artificial miRNAs were designed using natural Arabidopsis thaliana miRNA-319a backbone to specifically inhibit MPG1 gene expression. These artificial miRNAs were ligated into plant expression pPS1 vector to create gene construct for transferring into rice plant via Agrobacterium tumefaciens. 18 lines of transgenic rice variety J02 were identified to carry pre-amiR-P1 and these lines showed adaptation and survival under the greenhouse condition.

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