Date Received: 12-06-2014
Date Accepted: 20-09-2014
Date Published: 06-08-2025
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.8.
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Biological Characteristics of Race VD111 of Streptomyces albogriseolus
,
Pham Thanh Huyền
,
Le Gia Hy
,
Phi Quyet Tien
,
Ho Tuyen
,
Nguyen Van Giang
,
Nguyen Phuong Nhue (*)
Keywords
Antimicrobial activity, antibiotic, biological characteristics, marine actinomycet, Streptomyces albogriseolus VD111
Abstract
The inappropriate use of antibiotics might cause the development and wide spread of drug-resistance. Therefore, finding new antibiotics from marine microorganisms has been receiving attention by the scientists. This article presents the taxonomical characterization and antimicrobial activity of marine actinomycet strain VD111 isolated from Vietnam’s seawater. Based on Bergey’s classification and method of International Streptomyces Project (ISP), the strain VD111 was classified as Streptomyces albogriseolus since it had biological characteristics similar to the standard strain Streptomyces albogriseolus ISP 5003. The favourable conditions for the growth of the strain VD111 were established; temperature of 370C, pH of 7-8 and NaCl of 7-10%. The suitable medium and fermentation conditions for producing antimicrobial substances by the strain VD111 were A4H medium with the following substances expressed in g/l: 15g glucose, 15g soybean powder, 5g NaCl, and 1g CaCO3 and, pH=7. The preculture supplement was of 5.0% (v/v) and inoculum age of 48 hours. Maximal antibacterial compound yield (diameter of inhibition zone against Bacillus subtillis ATCC 6633: 30,2 mm) and biomass (10,6 mg/ml) were achieved after 60 hours of fermentation. Especially, the bioactive compound from strain Streptomyces albogriseolus VD111 exhibited antimicrobial activity against gram-positive, gram-negative bacteria and yeast (Candida albicans ATCC 10231).
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