Transformation of Sugarcane (Saccharum officinarum L.) with Insect Resistance Genes cry1ab, cry1b-cry1ab

Date Received: 25-07-2014

Date Accepted: 14-10-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Loc, P., Duong, H., Trường, M., Duc, L., Ha, T., Thanh, V., … Ho, N. (2025). Transformation of Sugarcane (Saccharum officinarum L.) with Insect Resistance Genes cry1ab, cry1b-cry1ab. Vietnam Journal of Agricultural Sciences, 12(7), 1058–1067. https://doi.org/10.31817/tckhnnvn.2014.12.7.

Transformation of Sugarcane (Saccharum officinarum L.) with Insect Resistance Genes cry1ab, cry1b-cry1ab

Phan Tuong Loc (*) , Hoang Van Duong , Mai Trường , Le Tan Duc , Tran Thi Ngoc Ha , Van Dac Thanh , Pham Duc Tri , Nguyen Thi Thanh , Nguyen Huu Ho

  • Tác giả liên hệ: [email protected]

    • Keywords

    transformation, sugarcane, Agrobacterium tumefaciens, cry1Ab, cry1B-cry1Ab, insect resistance

    Abstract


    The sugarcane variety VN84-4137 that was successfully confirmed with preliminary transformation experiments was further transformed with  Bt genes including a single synthetic gene cry1Ab and a hybrid synthetic gene cry1B-cry1Ab. After infection and co-culture with Agrobacterium, sugarcane calli were selected on callus induction medium with 3 mg/l phosphinothricin (PPT). Resistant calli were transferred to selective regeneration medium for shoot induction. Three cry1Ab lines and one cry1B-cry1Ab transgenic line were identified by PCR and Southern blot. Protein expression of Cry1Ab and Cry1B-Cry1Ab assayed by quick test kit gave positive results. Transgenic plants exhibited a normal phenotype.

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