Studies on Induction and Multiplication of Somatic Embryos of Ngoc Linh Ginseng (Panax vietnamensis Ha et Grushv.) in Liquid Medium

Date Received: 07-08-2014

Date Accepted: 13-10-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Truong, M., Ha, T., Tuan, T., Loc, P., Giap, D., Thach, B., … Ho, N. (2025). Studies on Induction and Multiplication of Somatic Embryos of Ngoc Linh Ginseng (Panax vietnamensis Ha et Grushv.) in Liquid Medium. Vietnam Journal of Agricultural Sciences, 12(7), 1085–1095. https://doi.org/10.31817/tckhnnvn.2014.12.7.

Studies on Induction and Multiplication of Somatic Embryos of Ngoc Linh Ginseng (Panax vietnamensis Ha et Grushv.) in Liquid Medium

Mai Truong , Tran Thi Ngoc Ha , Tran Trong Tuan , Phan Tuong Loc , Do Dang Giap , Bui Dinh Thach , Nguyen Thi Ngoc Han , Pham Duc Tri , Le Tan Duc , Nguyen Duc Minh Hung , Nguyen Van Ket , Nguyen Huu Ho (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.), somatic embryo induction, somatic embryo multiplication

    Abstract


    This paper presented results of induction and multiplication of somatic embryos of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) in liquid media. Leaf explants of in vivo plants were cultured on MS agar medium supplemented with 1 mg/l 2,4-D + 0.2 mg/l kinetin for the induction of friable calli. The friable calli were cultured in MS liquid medium supplemented with 1 mg/l 2,4-D + 0.2 mg/l kinetin + 500 mg/l casein hydrolysate for the formation of a cell suspension. This cell suspension was cultured in a B5 liquid medium with 3 mg/l IBA for the formation of a globular somatic embryo suspension. Embryonic cotyledon explants and intact immature somatic embryos from this suspension were cultured on MS agar medium containing 10% (v/v) coconut water with/without 0.2 mg/l IBA for the induction of secondary embryogenic calli and somatic embryos. These embryogenic calli and somatic embryos were also cultured in liquid media with or without plant growth regulators in conical flasks and in bioreactors. The results have laid the foundations for large-scale plant micropropagation and production of secondary metabolites.

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