Date Received: 12-11-2025
Date Accepted: 30-12-2025
Date Published: 23-01-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.1.02
Views
Downloads
How to Cite:
In vitro Propagation for Hippeastrum equestre (Aiton) Herb.
,
Nguyen Anh Duc
2
,
Nguyen Thi Thuy Hanh
3
,
Dinh Thai Hoang
,
Nguyen Viet Long (*)
4
Keywords
Hippeastrum equestre (Aiton) Herb., rooting, micropropagation, Vietnam
Abstract
The study aimed to complete an in vitro propagation protocol for Hippeastrum equestre (Aiton) Herb. The in vitro propagation experiments were carried out on MS media containing 30 g/l sucrose, 6 g/l agar, 0.5 g/l active charcoal with different concentrations of 6-Benzylaminopurine (BA), 2,4-dichlorophenoxyacetic (2,4-D), α-Napthaleneacetic Acid (α-NAA), and Indole-3-Acetic Acid (IAA). The results showed that plant material treated with Presept at 5 g/l for 50 minutes achieved the highest survival rate (90%). Shooting medium supplemented with 3 mg/l BA and 1.0 mg/l 2,4-D gained the highest shooting ability. Rooting medium supplemented with 1 mg/l α-NAA or 1.5 mg/l IAA was optimal for the highest rooting ability, with a rooting rate of 100% and number of roots/explant ranging from 3.33 to 4.00. In the nursery, coir : soil : organic fertilizer substrate with a ratio of 6:3:1 (v/v) yielded the highest survival rate (100%), leaf number (3.53 leaves/plant), and plant height (16.34 cm). The result could be utilized for large-scale propagation of Hippeastrum in Vietnam.
References
Chen X., Chen C., Zheng J., Lin, J., Wu P. & Ji Y. (2025). The tissue culture technology of Hippeastrum vittatum. Journal of Fujian Forestry Science and Technology. 52(2): 66-69.
De Buruyn M.H., Ferreira D.I., Slabbert M.M. & Pretorius J. (1992). In vitro propagation of Amaryllis belladonna. Plant Cell, Tissue and Organ Culture. 31: 179-184.
Đinh Trường Sơn, Bùi Huy Hoàng, Nguyễn Hải Ninh, Ninh Thị Phíp, Phạm Ngọc Khánh, Đặng Thị Thanh Tâm, Nguyễn Thị Lâm Hải & Nguyễn Thanh Hải (2021). Tạp chí Khoa học Nông nghiệp Việt Nam. 19(3): 301-310.
Epharath J.E., Ben A., Baruchin F., Alekperov C., Dayan E. & Silerbush M. (2001). Various cutting methods for the propagation of Hippeastrum bulbs. Biotronics. 30: 75-83.
Huang C.L., Chang K.O. & Hiroshi O. (2005). In vitro morphogenesis from ovaries of Hippeastrum × Hybridum. Journal of the Faculty of Agriculture, Kyushu University. 50(1): 19-25.
Hussey G. (1975). Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae and Amaryllidaceae. Journal of Experimental Botany. 26: 253-262.
Janet E.A.S. & Bruce G.C. (1977). The in vitro propagation of amaryllis (Hippeastrum spp. Hybrids). In Vitro. 13: 831-836.
Kendon J.P., Rajaovelona L., Sandford H., Fang R., Bell J. & Sarasan V. (2017). Collecting near mature and immature orchid seeds for ex situ conservation: 'in vitro collecting' as a case study. Botanical Studies. 58: 34-34.
Martínez‑Peinado N., Cortes‑Serra N., Tallini L.R., Maria‑Jesus P., Gascon J., Jaume Bastida J. & Alonso‑Padilla J. (2021). Amaryllidaceae plants: a potential natural resource for the treatment of Chagas disease. Parasi Vectors. 14: 337.
Mihaljević I., Dugalić K., Tomaš V., Viljevac M., Pranjić A., Čmelik Z., Puškar B. & Jurković Z. (2013). In vitro sterilization procedures for micropropagation of ‘Oblačinska’ sour cherry. Journal of Agricultural Sciences. 58: 117-126.
Murashige T. & Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Ngô Minh Trí, Trần Lê Nguyên, Trần Thị Mỹ Tiên, Lâm Ngọc Kim Trúc, Phùng Hoàng Yến, Dương Nguyễn Mai Anh, Phùng Thị Hằng & Nguyễn Thị Pha (2023). Khảo sát ảnh hưởng của 2,4-dichlorophenoxyacetic acid, α-naphthylacetic acid đến sự phát sinh biến dị trên lan Cẩm Cù (Hoya kerrii). Tạp chí Khoa học và Công nghệ Việt Nam. 65(9): 63-68.
Nguyen T.M.D, Trinh T.T.H, Diep N.T.H & Bui P.T. (2025). Study on micropropagation and acclimatization of hippeastrum ‘Double king’ flowers in An Gian province of Vietnam. Plant Science Today. 12(4): 1-8.
Ninh Thị Thảo, Nguyễn Thị Cúc, Nguyễn Hạnh Hoa & Nguyễn Thị Phương Thảo (2009). Bước đầu nghiên cứu quy trình nhân nhanh in vitro cây Loa kèn đỏ nhung (Hippeastrum equestre Herb.). Tạp chí Khoa học và Phát triển. 7(4): 453-459.
Phạm Đức Trọng, Nguyễn Hạnh Hoa & Phí Thị Cẩm Miện (2014). Nghiên cứu xây dựng quy trình nhân nhanh in vitro sáu dòng hoa Lan huệ - Hispeatrum esquestre (Aition) Herb. Tạp chí Khoa học và Phát triển. 12(3): 392-403.
Phạm Thị Minh Phượng & Trần Thị Minh Hằng (2014). Nghiên cứu kỹ thuật nhân giống vô tính cây lan huệ (Hippeastrum sp.) bằng phương pháp chẻ củ. Tạp chí Nông nghiệp và Phát triển nông thôn. 1(5): 32-39.
Siddique M.N.A, Sultana J., Sultana N. & Hossain M.M. (2007). Ex vitro establishment of in vitro produced plantlets and bulblets of Hippeastrum (Hippeastrum hybridum). International Journal of Sustainable Crop Production. 2(3): 22-24.
Srivastava S. & Dwivedi U.N. (2001). Plant regeneration from callus of Cuscuta reflexa - an angiospermic parasite -and modulation of catalase and peroxidase activity by salicylic acid and naphthalene acetic acid. Plant Physiology and Biochemistry. 39: 529-538.
Tang J., Li Y., Zhang L., Mu J., Jiang Y., Fu H., Zhang Y., Cui H., Yu X. & Ye Z. (2023). Biosynthetic pathways and functions of indole-3-acetic acid in microorganisms. Microorganisms, 11(8): 2077. https://doi.org/10.3390/microorganisms11082077.
Vijverberg A.J. (1981). Growing Amaryllis. Grower Book, London. p. 57.