Date Received: 15-03-2026
Date Accepted: 03-07-2026
Date Published: 27-08-2026
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.8.06
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Effects of Salinity and Microalgal Diets on Reproductive Performance of Broodstock Solen grandis under Controlled Conditioning
,
Kim Van Van
3
,
Nguyen Xuan Thanh (*)
Keywords
Solen grandis, broodstock conditioning, salinity, microalgae diet, artificial reproduction
Abstract
This study evaluated the effects of salinity and diet on the reproductive performance of broodstock razor clam (Solen grandis) under conditioning conditions to determine optimal parameters for artificial seed production. Two single-factor experiments were conducted using a completely randomized design (CRD), including four salinity treatments (15, 20, 25, and 30‰) and five dietary treatments (three single-species microalgae, a mixed diet at a 1:1:1 ratio, and a commercial algal diet), each with three replicates. Broodstock were conditioned for 30-45 days under controlled conditions, and spawning was induced by combined thermal and salinity shocks. Results showed that a salinity of 25‰ and a mixed live microalgal diet yielded the highest reproductive performance, with survival rates of 73.67 ± 2.89% and 77.30 ± 2.89%, spawning participation rates of 67.02 ± 3.13% and 70.48 ± 3.43%, fertilization rates of 76.00 ± 4.36% and 76.50 ± 2.60%, and D-larval rates of 69.47 ± 4.27% and 69.60 ± 4.13%, respectively. Realized fecundity reached 82.32 ± 2.11 × 104 and 79.56 ± 5.56 × 104 eggs per female (P >0.05). The commercial algal mixture could temporarily serve as a substitute for live microalgae. These findings identified salinity of 25 ± 1‰ and mixed live microalgae as suitable conditions for broodstock conditioning and artificial seed production.
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