Application of Somatic Embryogenesis and Ex Vitro Stem Cutting Technology in Propagation of Piper nigrum L.

Date Received: 25-07-2014

Date Accepted: 20-09-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Tram, T., Han, H., Linh, N., Cuong, H., Chien, H., Ton, L., & Nhut, D. (2025). Application of Somatic Embryogenesis and Ex Vitro Stem Cutting Technology in Propagation of Piper nigrum L. Vietnam Journal of Agricultural Sciences, 12(7), 988–995. https://doi.org/10.31817/tckhnnvn.2014.12.7.

Application of Somatic Embryogenesis and Ex Vitro Stem Cutting Technology in Propagation of Piper nigrum L.

To Thi Nha Tram , Ho Ngoc Han , Nguyen Thi Kim Linh , Hoàng Van Cuong , Hoang Xuan Chien , Le Dinh Ton , Duong Tan Nhut (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    BA, cutting, embryogenesis, Piper nigium L., TDZ

    Abstract


    This study examined the application of somatic embryogenesis and ex vitro stem cutting techniques for production of high quality and virus-free Piper nigrum planting materials. The results indicated that MS medium supplemented with 0.05 mg/l TDZ and 1.0 mg/l IBA was best for embryo induction with a rate of 73.2% and MS medium containing 3 mg/l BA gave the highest rate of shoot forrmation (66,7%) from leaf callus after 60 and 28 days of culture, respectively. Cuttings taken from ex vitro plants produced healthy root system when watered 6 times daily. Plants raised from these cuttings thrived best when misted 3 times a day.

    References

    Benelli C., Fabbri A., Grassi S., Lambardi M. and Rugini E. (2001). Histology of somatic embryogenesis in mature tissue of olive (Olea europaea L.). J. Hort. Sci. Biot., 76: 112-119.

    Bhat S.P., Chandel K.P.S. and Malik S.K. (1995). Plant regeneration from various explants of cultivated piper species. Plant Cell Rep., 14: 398-402.

    Duncan D.B. (1995) Multipe range and multiple F test. Biometrics 11: 1-42.

    Fuentes S.R.L., Calheiros M.B.P., Manetti-Filho J. and Vieira L.G.E. (2000). The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss. Org. Cult., 60: 5-13.

    Gairi A. and Rashid A. (2004). TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2,4-D. Plant Cell Tiss Org. Cult., 76: 29-33.

    Gill R. and Saxena P.K. (1993). Somatic embryogenesis in Nicotiana tabacum L.: Induction by TDZ of direct embryo differentiation from cultured leaf discs. Plant Cell Rep., 12: 154-159.

    Gill R., Gerrath J.M. and Saxena P.K. (1993). High-frequency direct somatic embryogenesis in thin layer cultures of hybrid seed geranium (Pelargonium x hortorum). Can. J. Bot., 71: 408-413.

    Gumerova E.A., Galeeva E.I., Chuyenkova S.A. and Rumyantseva N.I. (2003). Somatic embryogenesis and bud formation on cultured Fagopyrum esculentum hypocotyls. Russian J. Plant Physiol., 50: 640-645.

    Joseph B., Joseph D. and Philip V.J. (1996). Plant regeneration from somatic embryos in black pepper. Plant Cell Tiss. Organ Cult., 47:87-90.

    Kharwar K,M. (2003) Effects of thidiazuron on shoot regeneration from different explants of Lentil (Lens culinaris Medik.) via organogenesis. Turk. J. Bot., 28:421-426.

    Kintzios S., Sereti E., Bluchos P., Drossopoulos J.B., Kitsaki C.K. and Liopa-Tsakalidis A. (2002). Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo L.) and melon (Cucumis melo L.). Plant Cell Rep., 21: 1-8.

    Litz R.E. and Gray D.J. (1995). Somatic embryogenesis for agricultural improvement. World J. Microbiol. Biotechnol., 11: 416-425.

    Murashige T. and Skoog F. (1962). Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473-479.

    Murthy B.N.S., Murch S.J. and Saxena P.K. (1995). Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons. Plant Physiol., 94: 268-276.

    Nair R.R. and Gupta S.D. (2006). High-frequency plant regeneration through cyclic secondary somatic embryogenesis in black pepper (Piper nigrum L.). Plant Cell Rep., 24: 699-707.

    Philip V.J., Joseph D., Triggs G.S. and Dickinson N.M. (1992). Micropropagation of black pepper (Piper nigrum Linn) through shoot tip cultures. Plant Cell Rep., 12: 41-44.

    Umehara M. and Kamada H. (2005). Development of the embryo proper and the suspensor during plant embryogenesis. Plant Biot., 22: 253-260.

    Visser C., Qureshi J.A., Gill R. and Saxena P.K. (1992). Morpho-regulatory role of thidiazuron substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in Geranium hypocotyl cultures. Plant Physiol., 99: 1704-1707.

    Wang Y.H. and Bhalla P.L. (2004). Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br. Plant Cell Rep., 22: 408-414.