Ngày nhận bài: 31-07-2025
Ngày xuất bản: 06-08-2025
Lượt xem
Download
Cách trích dẫn:
IMMUNO-RELATED GENE EXPRESSION OF ANTI-LIPOPOLYSACCHARIDE FACTORS (ALF) AFTER USING IMMUNOSTIMULANT IN KURUMA SHRIMP (Marsupenaeus japonicus)
,
Lai Thi Lan Huong
1
Từ khóa
Anti-lipopolysaccharide factor (ALF), gene expression, immunostimulants, Kuruma shrimp, quantitative real-time PCR
Tóm tắt
Supplementation of immuno-stimulant can act as a potent immune modulator and have the effect on inducion of immune- related genes which involved in host defense. In order to clarify the anti-lipopolysaccharide factors (ALF) expression of Kuruma shrimp (Marsupenaeus japonicus) after feeding immuno-stimulant, the supplement containing microalgae and Euglena (Ex400) was selected for the trials. Total RNA was extracted by RNA Iso Plus from the lymphoid organ, intestine and blood of shrimp that was fed with Ex400 diet. Tissues were collected at 0, 3, 7, and 10 days after feeding. ALF transcripts were significantly increased by Ex400 compared to control-diet-fed shrimp (with P<0.01) at 3 days post-feeding in the intestine and lymphoid organ. This suggested Ex test diet might stimulate ALF of Kuruma shrimp immune defence in the intestine and lymphoid organ.
Tài liệu tham khảo
Bachère, E., Gueguen, Y., Gonzalez, M., De Lorgeril, J., Garnier, J., & Romestand, B. (2004). Insights into the antimicrobial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunological reviews,
(1): 149-168.
Beale, K., Towle, D., Jayasundara, N., Smith, C., Shields, J., Small, H., & Greenwood, S. (2008). Anti-lipopolysaccharide factors in the American lobster Homarus americanus: Molecular characterization and transcriptional response to Vibrio fluvialis challenge. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 3(4): 263-269.
Boonyaratpalin, S., Boonyaratpalin, M., Supamattaya, K., & Toride, Y. (1995). Effects of peptidoglycan (PG) on growth, survival, immune response, and tolerance to stress in black tiger shrimp, Penaeus monodon. Diseases in Asian Aquaculture II. Fish Health Section. Manila, Philippines: Asian Fisheries Society, pp. 469-477.
Burgents, J. E., Burnett, L. E., Stabb, E. V., & Burnett, K. G. (2005). Localization and bacteriostasis of Vibrio introduced into the Pacific white shrimp, Litopenaeus vannamei. Developmental & Comparative Immunology, 29(8): 681-691.
El-Asely, A., Shaheen, A., Abbass, A., Inada, M., Okugawa, S., Tachikawa, Y., . . . Itami, T. (2011). Changes in the immune-related gene expression of kuruma shrimp Marsupenaeus japonicus in response to dietary inclusion of Macrophage Activating Chinese Herbs (MACH). Paper presented at the Proceedings of the 4th Global Fisheries and Aquaculture Research Conference, the Egyptian International Center for Agriculture, Giza, Egypt, 3-5 October 2011.
Jayabalan, N., Chandran, R., Sivakumar, V., & Ramamoorthi, K. (1982). Occurrence of luminescent bacteria in sediments: Current science Assn CV raman avenue, Po Box 8005, Bangalore 560 080, India. 51: 710-711.
Johnson, P. T. (1987). A review of fixed phagocytic and pinocytotic cells of decapod crustaceans, with remarks on hemocytes. Developmental & Comparative Immunology, 11(4): 679-704.
Liuxy, P. C., Lee, K. K., & Chen, S. N. (1996). Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Letters in applied microbiology, 22(6): 413-416.
Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25(4): 402-408.
Maeda, M., Shibata, A., Biswas, G., Korenaga, H., Kono, T., Itami, T., & Sakai, M. (2014). Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. Marine biotechnology, 16(2): 181-192.
Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., Sakai, M., & Itami, T. (2010). Molecular cloning and transcriptional analysis of a newly identified antilipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus. Letters in applied microbiology, 50(1): 112-119.
Miyata, T., TOKUNAGA, F., NAKAMURA, T., & IWANAGA, S. (1987). Primary structure of anti-lipopolysaccharide factor from American horseshoe crab, Limulus polyphemus. Journal of biochemistry, 101(6): 1321-1330.
Oka, M. (1969). Studies on Penaeus orientalis Kishinouye-VIII. Structure of the newly found lymphoid organ. Bull Jpn Soc Sci Fish, 35: 245-250.
Raa, J. (1996). The use of immunostimulatory substances in fish and shellfish farming. Reviews in Fisheries Science, 4(3): 229-288.
Selsted, M. E., & Ouellette, A. J. (2005). Mammalian defensins in the antimicrobial immune response. Nature immunology, 6(6): 551-557.
Sordillo, L., Shafer-Weaver, K., & DeRosa, D. (1997). Immunobiology of the mammary gland. Journal of dairy science, 80(8): 1851-1865.
Tanekhy, M., & Fall, J. (2015). Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin). Veterinarni Medicina, 60(1): 39-47.
Van de Braak, C., Botterblom, M., Taverne, N. v., Van Muiswinkel, W., Rombout, J., & Van der Knaap, W. (2002). The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp. Fish & shellfish immunology, 13(4): 293-309.