Micropropagation of Four Economically Valuable Cymbidium Species

Date Received: 07-08-2014

Date Accepted: 13-10-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Lan, T., Hoang, N., Sanh, N., & Nhut, D. (2025). Micropropagation of Four Economically Valuable Cymbidium Species. Vietnam Journal of Agricultural Sciences, 12(7), 1125–1133. https://doi.org/10.31817/tckhnnvn.2014.12.7.

Micropropagation of Four Economically Valuable Cymbidium Species

Tran Thi Ngoc Lan , Nguyen Hong Hoang , Nguyen Du Sanh , Duong Tan Nhut (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Apical meristem, Cymbidium, thin cell layers, PLBs

    Abstract


    Protocorm-like bodies (PLBs) were formed from apical meristem culture of four Cymbidium species on MS medium supplemented with 0.5 mg/l αNAA., 1 mg/l BA after 45 days under light condition. PLB longitudinal thin cell layer culture was used as a technique for PLBs micropropagation on MS medium containing 0.5 mg/l αNAA, 1 mg/l BA, 10% coconut water (v/v), 30 g/l sucrose, 0.2 g/l glutamine, 1 g/l activated charcoal and 8 g/l agar. These collected PLBs were maintained and proliferated on the same medium after 2 months and then transferred to MS medium supplemented with 0.5 mg/l αNAA, 1 g/l activated charcoal, 30 g/l sucrose, 10% coconut water (v/v) and 8 g/l agar for plantlet regeneration. Histological observation proved that these PLBs originated from somatic embryos. Plantlets regenerated on medium without plant growth regulators after 4 months resulted in well-developed shoots and roots. This investigation is important for micropropagation and preservation of the high-economic Cymbidiums.

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