Genetic Diversity Among 25 Accessions of Camellia spp. Collected in Quang Ninh by RADP and ISSR markers

Date Received: 24-05-2017

Date Accepted: 19-09-2017

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2017.15.8.

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Issue: Vol. 15 No. 8 (2017)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Bich, D., Thao, N., Phu, N., Thao, N., Ha, H., Ha, D., … Son, D. (2025). Genetic Diversity Among 25 Accessions of Camellia spp. Collected in Quang Ninh by RADP and ISSR markers. Vietnam Journal of Agricultural Sciences, 15(8), 1077–1092. https://doi.org/10.31817/tckhnnvn.2017.15.8.

Genetic Diversity Among 25 Accessions of Camellia spp. Collected in Quang Ninh by RADP and ISSR markers

Dang Quang Bich , Nguyen Thi Phuong Thao , Nguyen Van Phu , Ninh Thi Thao , Hoang Hai Ha , Doan Thi Thu Ha , Nguyen Thi Thuy Linh , Dinh Truong Son (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Golden flower tea plant, Camellia spp., ISSR, RAPD, genetic diversity

    Abstract


    Golden flower tea plant (Camellia spp.) is one of the important medicinal plant. Genetic diversity among 25 accessions of Camellia spp., collected mostly in Quang Ninh, were evaluated by RAPD and ISSR molecular markers. Using 53 molecular markers (45 RAPD and 8 ISSR marker), we detected 294 loci with total 4656 clear and scorable bands, among these, 3628 were polymorphic bands. Based on a total of 4656 bands and similarity coefficient ranged from 0.54 to 0.81, dendrogram analysis was performed. With the genetic similarity at 0.71, 25 Camellia spp. accessions could be divided in to 9 distinct clusters. The combination of phenotypic analysis, genetic variation as well as analysis of pharmacologically active compounds showed that amongs 25 golden flower tea accessions, although they look similar, however, quite diverse in genotype and the ability to accumulate pharmacological compounds. The collected accession will be a valuable source for breeding programs of golden flower tea plants in Quang Ninh.

    References

    Zhang Ang, Wan Li, Wu Cuiyun, Fang Yulin, Han Guomin, Li Hua, Zhang Zhenwen and Wang Hua (2013). Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection. Molecules, 18: 14241-14257.

    Bhagyawant S. S., Srivastava N. (2008). Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships. Afr. J. Biotechnol., 7(24): 4428-4431.

    Carvalho A., Matos M., Lima-Brito J., Guedes-Pinto H., Benito C. (2005). DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs. Euphytica, 143(1-2): 93-99.

    Chen, Y.Y, Huang, Y.L, Wen, Y.X, Li, D.P, Liu, J.L, Wei, X. (2009). Analysis of volatile components in Camellia nitidssima by GC-MS, Guangxi Institute of Botany, Guilin 541006

    Fouly H.M., Wilkinson H.T., Domier L.L (1996). Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species. Soil Biol. Biochem., 28(6): 703-710.

    Guasmi F., Elfalleh W., Hannachi H., Fères K., Touil L., Marzougui N., Triki T and Ferchichi A. (2012). The use of ISSR and RAPD markers for genetic diversity among South Tunisian barley. ISRN Agronomy. Article ID 952196.

    Hoàng Chung (2006). Các phương pháp nghiên cứu quần xã thực vật. Nhà xuất bản Giáo Dục.

    Hoàng Thị Sản, Hoàng Thị Bé (2005). Phân loại thực vật học. Nhà xuất bản Đại học Sư phạm, tr. 18-19; 297-303.

    Hoàng Thị Thu Yến, Nguyễn Văn Tuấn, Hoàng Thị Ngà (2012). Nghiên cứu đa dạng di truyền genome một số dòng chè (Camellia siensis) trồng tại xã Tân Cương, thành phố Thái Nguyên bằng kỹ thuật RAPD, Tạp chí Khoa học & Công nghệ, 96(08): 139-143.

    Kawa P.G., Devarumath R.M., Nerka Y (2009). Use of RAPD marker for assessment of genetic diversity in sugarcane cultivars. Indian J. Biotechnol., 8: 67-81.

    Khan A., Awan S., Sadia B., Rana R. M Khan I. A (2010). Genetic diversity study among coloured cotton genotypes by usin RAPD markers. Pak. J. Bot., 42(1): 71-77.

    Lin S.Y, Chen I.Z, Tsai C.M, Chen Y.L (2005). Detection of genetic relationship in Taiwan tea variety (Camellia sinensis (L.) O. Kuntze) with RAPD markers, Journal of the Chinese Society for Horticultural Science, 51(4): 357-366.

    Matsumoto S, Takeuchi A, Hayatsu M, Kondo S (1994). Molecular cloning of phenylalanine ammonialyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe, Theoretical and applied genetics, 89(6): 671-675.

    Martin C., Uberhuaga E., Pérez C. (2002). Application of RAPD markers in the characterisation of Chrysanthemum varieties and the assessment of somaclonal variation. Euphytica, 127(2): 247-253.

    Mishra P.K., Fox R.T.V., Culhamm A. (2003). Inter-simple sequence repeat and aggressiveness analyses revealed high genetic diversity, recombination and longrange dispersal in Fusarium culmorum. School of Plant Sciences, The University of Reading, Whiteknights, Reading RG6 6AS, UK, 2003 Association of Applied Biologists.

    Nguyễn Văn Mùi, Lê Thị Hoài Thu (2004). Nghiên cứu tính đa dạng di truyền của một số loài trà hoa vàng (Camellia) Việt Nam bằng kỹ thuật RAPD - PCR. Tạp chí Khoa học ĐHQGHN, KHTN & CN, XX(4).

    Ngô Quang Đê (1996). Nghiên cứu hai loài cây Camellia có triển vọng thuần hóa làm cây cảnh ở Ba Vì - Hà Tây, Báo cáo kết quả nghiên cứu khoa học, Trường đại học Lâm nghiệp.

    Oliveira E.C, Amaral Junior A.T, Goncalves L.S.A, Pena G.F, Freitas Junior S.P, Ribeiro R.M, Pereira M.G (2010). Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays). Genetic and Molecular Research, 9(2): 835-842.

    Qian W., Ge S., Hong D. Y. (2001). Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor. Appl. Genet., 102(2-3): 440-449.

    Reddy P.M., Sarla N., Siddiq E.A (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128(2): 9-17.

    Rossi. I., Bartolacci. B., Potenza. L., Bertini.

    L., Barbieri. E., Stocchi. V. (2000). Identification of white truffle species using RAPD markers. Plant and Soil, 219(1-2): 127-133.

    Sinchan Adhikari Soumen Saha Tapas Kumar Bandyopadhyay Parthadeb Ghosh (2014). Efficiency of ISSR marker for characterization of Cymbopogon germplasms and their suitability in molecular barcoding. Plant Systematics and Evolution, January 2014, Volume 301, Issue 1, pp 439-450

    Trần Ninh, Hakoda Naotoshi (2010). Các loài trà ở vườn Quốc gia Tam đảo, tr. 122-125.

    Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski A.J., and Tingey S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.

    Zheng Xiao, Zhi Hui-Li, Huang Dong-Lian, Zheng Qi-Fan, Chang Jie-Jiang (2013). Genetic Relationship among 29 Yellow Camellia Germplasms in Nanning Golden Park by ISSR Analysis. Research Institute of Subtropical, Forestry, Caf, Fuyang, 311400, Zhejiang, China; Nanning Golden Camellia Park, Nanning, Guangxi, 530023, China.