Screening Streptomyces sp.  Strains for Antagonistic Activity against Phytopathogenic Alternaria alternata

Date Received: 05-12-2025

Date Accepted: 04-01-2026

Date Published: 29-04-2026

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.4.07

Views

0

Downloads

0

How to Cite:

Tam, D., Thu, N., Thao, N., & Giang, N. (2026). Screening Streptomyces sp.  Strains for Antagonistic Activity against Phytopathogenic Alternaria alternata. Vietnam Journal of Agricultural Sciences, 24(4), 496–505. https://doi.org/10.31817/tckhnnvn.2026.24.4.07

Screening Streptomyces sp.  Strains for Antagonistic Activity against Phytopathogenic Alternaria alternata

Dang Thi Thanh Tam 1 , Nguyen Thi Thu 1 , Nguyen Vu Phuong Thao 1 , Nguyen Van Giang (*)

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    biological active compounds, pathogenic fungi, Streptomyces spp.

    Abstract


    This study was conducted with the aim of screening and evaluating the antifulgal activity against Alternaria alternata of potential native actinomycete genetic resources. The antagonistic activity of actinomycetes strains against A. alternata was evaluated through methods such as co-cultivation, agar diffusion analysis, and molecular techniques to identify genes involved in the synthesis of bioactive compounds in the selected actinomycete strains. Experimental results showed that, using co-cultivation on PDA medium, nine strains with antagonistic activity against A. alternata were screened from 56 actinimycete strains. Notably, Streptomyces sp. VNUA24 showed the strongest antifungal effect, with an inhibition percentage of 77.62 ± 2.63% after 12 days of  co-cultivation. The 7-day culture filtrate of the strain VNUA24 grown in Gause I medium did not display any antagonistic activity against A. alternata. Interestingly, evaluation of the volatile organic compounds produced by Streptomyces sp. VNUA24 revealed inhibitory effects, resulting in an antagonistic activity of 23.13% after 7 days of incubation. The PCR analysis detected the presence of 5 out of 8 biosynthetic genes associated with the production of bioactive metabolites belonging to the PKS-I, PKS-II, and NRPS gene clusters. Overall, the findings indicate that Streptomyces sp. VNUA24 is a promising candidate for the development of biocontrol products in managing crop diseases caused by A. alternata. 

    References

    Ahmad T., Xing F., Cao C. & Liu Y. (2024). Characterization and toxicological potential of Alternaria alternata associated with post-harvest fruit rot of Prunus avium in China. Frontiers in Microbiology. 15: 1273076. Arrebola E., Sivakumar D. & Korsten L. (2010). Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus. 53(1): 122-128. Ayed A., Kalai-Grami L., Ben Slimene I., Chaouachi M., Mankai H., Karkouch I., Djebali N., Elkahoui S., Tabbene O., Limam F. (2021). Antifungal activity of volatile organic compounds from Streptomyces sp. strain S97 against Botrytis cinerea. 31(12): 1330-1348. Bai Q., Ma X., Hayat M., Tang Y. & Wang Z. (2025). Comparison and Analysis of Resistance Differences in Alternaria alternata from Fungicides with Three Different Mechanisms. Journal of Fungi. 11(4): 305. Beroigui O. & Errachidi F. (2023). Streptomyces at the heart of several sectors to support practical and sustainable applications: A review. 6(1). Bundale S., Begde D., Nashikkar N., Kadam T. & Upadhyay A. (2014). Isolation of aromatic polyketide producing soil Streptomyces using combinatorial screening strategies. OALib J. 1: 1-16. Cai L., Zhang H., Deng Y., Tian W., Fan G. & Sun X. (2023). Antifungal Activity of Streptomyces hygroscopicus JY-22 against Alternaria alternata and Its Potential Application as a Biopesticide to Control Tobacco Brown Spot. Agronomy. 13(7): 1944. Coakley S.M., Scherm H. & Chakraborty S. (1999) Climate change and plant disease management. Annu. Rev. Phytopathol. 37: 399-426. Dhanasekaran D., Thajuddin N. & Panneerselvam A. (2012). Applications of actinobacterial fungicides in agriculture and medicine. Fungic Plant Anim. 2: 29-54.

    Đỗ Quang Trung, Nguyễn Thị Thu Hằng, Phạm Bích Ngọc, Đinh Mai Vân, Trần Thị Hằng & Lưu Thế Anh (2021). Nghiên cứu in vitro các yếu tố ảnh hưởng đến sự phát triển của nầm Alternaria alternata gây bệnh thối ngọn cành trên cây thanh long (Hylocereus spp.). Tạp chí Khoa học Đại học Cần Thơ. 57(2): 115-120. Evangelista-Martínez Z., Contreras-Leal E.A., Corona-Pedraza L.F. & Gastélum-Martínez É. (2020). Biocontrol potential of Streptomyces sp. CACIS-1.5CA against phytopathogenic fungi causing postharvest fruit diseases. Egypt J. Biol. Pest Control. 30: 117. Gallo A., Ferrara M. & Perrone G. (2013). Phylogenetic Study of Polyketide Synthases and Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Mycotoxins. Toxins. 5(4): 717-742. Ghanem G.A.M., Gebily D.A.S., Ragab M.M. Ayat M., Ali. A., Soliman K.N. & Abd ElMoity H. (2022). Efficacy of antifungal substances of three Streptomyces spp. against different plant pathogenic fungi. Egypt J. Biol. Pest Control. 32: 112

    Hồ Thị Cẩm Nguyên, Đoàn Cẫm Tiên, Lê Minh Trường, Trịnh Minh Hợp & Nguyễn Thị Nhã (2024). Nghiên cứu tác nhân gây bệnh đốm lá và bệnh teo đầu lá nha đam (Aloe vera) trồng tại tỉnh Ninh Thuận. Tạp chí Nông nghiệp và Môi trường. 3+4: 86-92

    Khushboo Kumar P., Dubey K.K., Usmani Z., Sharma M. & Gupta V.K. (2022). Biotechnological and industrial applications of Streptomyces metabolites. 16(1): 244-264 Komaki H. & Tamura T. (2023). Profile of PKS and NRPS Gene Clusters in the Genome of Streptomyces cellostaticus NBRC 12849T. Fermentation. 9(11): 924. Liu Y., Bao M., Wang Y. & Zhang C. (2025). Development of Procymidone and Difenoconazole Resistance in Alternaria alternata, the Causal Agent of Kiwifruit Brown Spot Disease. Plants. 14(14): 2245. Masoomi-Aladizgeh F., Jabbari L., Khayam Nekouei R. & Aalami A. (2016). A simple and rapid system for DNA and RNA isolation from diverse plants using handmade kit. Protoc Exch. Miranda-Apodaca J., Artetxe U., Aguado I., Martin-Souto L., Ramirez-Garcia A., Lacuesta, M., Becerril J.M., Estonba A., Ortiz-Barredo A., Hernández A., Zarraonaindia I. & Pérez-López, U. (2023). Stress Response to Climate Change and Postharvest Handling in Two Differently Pigmented Lettuce Genotypes: Impact on Alternaria alternata Invasion and Mycotoxin Production. Plants (Basel, Switzerland): 12(6): 1304.

    Nguyễn Thị Liên, Nguyễn Nhựt Hào, Nguyễn Lam Minh & Nguyễn Tăng Phú (2024). Phân lập và tuyển chọn vi khuẩn đối kháng nấm Alternaria Alternata gây bệnh đốm lá trên dưa leo. Tạp chí Khoa học Nông nghiệp Việt Nam. 22(2): 244-251.

    Nguyễn Thị Thanh Mai, Nguyễn Thị Thu, Trần Văn Tuấn, Phạm Hồng Hiển & Nguyễn Xuân Cảnh (2023). Khảo sát một số đặc điểm sinh học của chủng xạ khuẩn Streptomyces diastatochromogenes VNUA27 sử dụng trong kiểm soát nấm bệnh hại cây chuối. Tạp chí Khoa học Công nghệ Việt Nam (Bản B): 65(5): 59-63

    Nguyễn Xuân Trường, Nguyễn Thị Thu, Võ Thị Bích Thủy, Đặng Thị Thanh Tâm, Nông Thị Huệ, Phạm Hồng Hiển & Nguyễn Xuân Cảnh (2024). Nấm Alternaria alternata gây bệnh đốm lá trên bắp cải (Brassica oleracea L. var. capitata) tại tỉnh Nghệ An. Tạp chí Khoa học Nông nghiệp Việt Nam. 22(1): 107-114. Paster N. & Barkai-Golan R. (2008). Mouldy fruits and vegetables as a source of mycotoxins: Part 2. World Mycotoxin J. 1(4): 385-396. Pipponzi S., Licciardello F., Caradonia F. & Stefani E. (2025). Volatile organic compounds produced by Streptomyces sp. SA51 mitigate Fusarium oxysporum f.sp. lactucae infection and stimulate lettuce seedling growth. Eur J. Plant Pathol. 173: 125-142 Qi D., Zou L., Zhou D., Chen Y., Gao Z., Feng R., Zhang M., Li K., Jianghui X. & Wang, W. (2019). Taxonomy and broad-spectrum antifungal activity of Streptomyces sp. SCA3-4 isolated from rhizosphere soil of Opuntia stricta. Frontiers in Microbiology. 10: 1390. Raynaldo F.A., Xu Y., Wang Q., Wu B. & Li D. (2024). Biological control and other alternatives to chemical fungicides in controlling postharvest disease of fruits caused by Alternaria alternata and Botrytis cinerea. Food Innovation and Advances. 3(2): 135-143. Tam T.T.D., Son T.D., Thu T.N., Hai T.N. & Canh X.N. (2023). Complete genome sequence of Streptomyces sp. strain VNUA24, a potential antifungal bacterium isolated from soil. National Library of Medicine. 12(9): e00372-23. Tenebro C.P., Trono D.J.V.L., Vicera C.V.B., Sabido E.M., Ysulat Jr J.A., Macaspac A.J.M., Tampus K.A., Fabrigar T.A.P., Saludes J.P & Dalisay D.S. (2021). Multiple strain analysis of Streptomyces species from Philippine marine sediments reveals intraspecies heterogeneity in antibiotic activities. Scientific reports. 11(1): 17544. Zhang F., Wen S., Wang B., Zhang Z., Liu F., Ye T., Wang K., Hu H., Yang X. & Fang W. (2023). Biocontrol Potential of Streptomyces odonnellii SZF-179 toward Alternaria alternata to Control Pear Black Spot Disease. 24(24): 17515. Zhang M.-J., Zheng X.-R., Li H. & Chen F.-M. (2023). Alternaria alternata, the Causal Agent of a New Needle Blight Disease on Pinus bungeana. Journal of Fungi. 9(1): 71. Zhang Y., Liu C. & van der Fels ‐ Klerx H.J. (2025). Occurrence, toxicity, dietary exposure, and management of Alternaria mycotoxins in food and feed: A systematic literature review. Compr Rev Food Sci Food Saf. 24(1): e70085.