Isolation and Selection of Bacillus amyloliquefaciens Strains Antagonistic Against Salmonella gallinarum

Date Received: 01-01-2026

Date Accepted: 30-12-2025

Date Published: 23-01-2026

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2026.24.1.07

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Issue: Vol. 24 No. 1 (2026): Tạp chí Khoa học Nông nghiệp Việt Nam

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Duc, H., Hang, N., Lan, N., Ha, C., Hoa, T., Linh, P., & Son, H. (2026). Isolation and Selection of Bacillus amyloliquefaciens Strains Antagonistic Against Salmonella gallinarum. Vietnam Journal of Agricultural Sciences, 24(1), 60–69. https://doi.org/10.31817/tckhnnvn.2026.24.1.07

Isolation and Selection of Bacillus amyloliquefaciens Strains Antagonistic Against Salmonella gallinarum

Hoang Minh Duc , Nguyen Thi Thu Hang 1 , Nguyen Thi Lan 1 , Cam Thi Thu Ha (*) , Tran Thi Khanh Hoa 1 , Pham Thuy Linh 1 , Hoang Minh Son 1

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Probiotics, antimicrobial resistance, salmonellosis

    Abstract


    Probiotics are one of the most popular alternatives to antibiotics for disease prevention and growth promotion in livestock. Bacillus amyloliquefaciens (B. amyloliquefaciens) possesses several characteristics suitable for probiotics, including resistance to high temperatures, low pH, bile salts, and antagonism against pathogens. This study was conducted to isolate and select B. amyloliquefaciens strains antagonistic against Salmonella gallinarum (S. gallinarum), which causes Salmonellosis. The result showed that four B. amyloliquefaciens strains were isolated and identified from 100 fresh fecal samples of healthy chickens using the culture methods and Matrix-Assisted Laser Desorption/Ionization - Time-of-Flight (MALDI-TOF) technology. The isolates were not hemolytic on blood agar and exhibited high stability in adverse conditions (pH 3.0 and 0.3% bile salt), with a survival rate greater than 80%. The results of the antimicrobial susceptibility test revealed that all 4/4 (100%) of the B. amyloliquefaciens isolates were resistant to tetracycline and 3/4 (75%) were resistant to erythromycin. The isolates also exhibited strong antagonism against S. gallinarum in vitro, producing inhibition zones with diameters greater > 16 mm.

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