Date Received: 15-06-2025 / Date Accepted: 10-04-2026 / Date Published: 29-04-2026
This study aimed to detect the presence of Gyrovirus galga 1 (GyVg1) in domestic dogs from several districts in Hanoi, Vietnam. A total of 63 fecal samples were collected from dogs raised he former Dong Anh, Long Bien, and Gia Lam districts between March 2024 and May 2025. Viral DNA was detected using polymerase chain reaction (PCR). The overall positive detection rate of GyVg1 was 12.7%, with rates of 14.29%, 12.12%, and 11.11% in Dong Anh, Long Bien, and Gia Lam districts, respectively. Partial sequences of the overlapping VP2 and VP3 genes from three representative strains were analyzed, revealing high nucleotide similarity rates ranging from 99.40% to 99.85%. Phylogenetic analysis demonstrated that these strains belong to Gyrovirus galga 1 and they clustered closely with strains previously reported in dogs and cats from China in 2022. Additionally, several amino acid substitutions were identified in the VP2 and VP3 proteins of the Vietnamese strains. These findings provide the first evidence of GyVg1 circulation in domestic dogs in Hanoi and lay the groundwork for future studies on the virus’s epidemiology, genetic diversity, and potential cross-species transmission.