Date Received: 24-11-2025 / Date Accepted: 03-02-2026 / Date Published: 28-02-2026
The study evaluated the in vitro regeneration of different mungbean varieties under different culture conditions and explant types. The study used 10 varieties and different explant types, concentrations of plant growth regulators, and kanamycin. The results showed that callus induction increased with higher BAP concentrations and was strongly enhanced when combined with NAA. Media containing 3-4 mg/l BAP +1.0 mg/l NAA produced the highest callus induction rates (73.2-75.3%), especially in shoot and hypocotyl explants (~ 50%), whereas leaf explants exhibited the lowest rate (28.7%) and were more susceptible to contamination (26.5%). In shoot regeneration from cotyledon, MS + 0.5-2.0 mg/l BAP + 0.1 mg/l IAA yielded the best results, with shoot induction rates of 80.0-82.2% and 0.8-1.7 shoots/explant. HMB0024 showed the best regeneration capacity (1.7 shoots/explant). Root formation on MS was 50.0-61.7% depending on the variety; HMB0036 produced the highest number of roots and root length (10.1 roots/explant; 9.0cm). Thus, the optimal medium for callus induction from shoots and hypocotyls was MS + 3-4 mg/l BAP + 1.0 mg/l NAA, while shoot regeneration from cotyledonary leaves was most effective on MS + 0.5-2.0 mg/l BAP + 0.1 mg/l IAA. Kanamycin concentrations of 75-100 mg/l were identified as suitable for screening cells, tissues or plants in genetice manipulation. The findings provide foudation for genetic transformation or genome editing in mungbean.
