Date Received: 07-02-2025 / Date Published: 21-02-2025
In this work, liquid chromatography in combination with tandem mass spectrometry (LC-MS/MS) was developed and applied to analyze biogenic amines in wine. Histamine, tyramine, tryptamine, phenylethylamine, putrescine, cadaverine, ethanolamine, and 5-hydroxyltryptamine were directly analyzed by LC-MS/MS using a HILIC column without a pre-column chemical derivatization step. Some critical parameters affecting the selectivity and sensitivity of method such as selection of precursor and product ions, collision energy, component of mobile phase… were investigated and optimized. Linearity range was established from 5 to 7.000 µg/l association with regression coefficient R2 > 0.995; The limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.4-31.0 µg/l and 1.3-103.3 µg/l, respectively. Relative standard deviation of repeatability and reproducibility were in the range of 1.3-4.8% and 2.8-5.0%, respectively. The recovery of 8 biogenic amines was from 80.2% to 100.6%. The validated LC-MS/MS method was then applied to analyze 8 biogenic amines in 5 wine samples collected on the local market. Experimental results indicated that total biogenic amines range from 1,088-3,394 µg/l in analyzed samples. Histamine, considered as the most toxic and food safety relevant, was presents in all analyzed samples. However, the concentration of this compound in all analyzed samples was below the safe level according to EFSA.