Date Received: 16-05-2025 / Date Accepted: 19-05-2025 / Date Published: 23-05-2025
Probiotics are known as a promising alternative to antibiotics in improving productivity and controlling infectious diseases in livestocks. The study was conducted to isolate lactic acid bacteria (LAB) with strong antagonism against some common pathogenic bacteria in livestocks. A total of 33 LAB strains were isolated from 100 chicken intestine and pork intestine samples. Among them, 5 isolates showing strong antagonistic activity against 5 pathogenic bacteria tested (S. Typhimurium, S. Gallinarum, E. coli K88, S. aureus, and C. perfringens) were identified as Pediococcus pentosaceus, Levilactobacillus brevis, Lactiplantibacillus plantarum using MALDI TOF. The LAB isolates in this study were non-hemolytic, negative for DNase production, and highly resistant to acid and bile salt. The auto-aggregation rates of the LAB isolates were all over 40% and the rates of co-aggregation of them to the pathogens ranged from 24.39% to 45.17%. In particular, all LAB isolates were determined as multi-resistant strains, with the highest resistant rates to ampicillin, erythromycin, and tetracycline. Overall, the 5 selected LAB isolates are potential strains for the production of probiotics preventing animal diseases.